Brg1 Antibody [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB, ICC/IF, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

Brg1 Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human Brg1
  Gln673-Asn774
  Accession # P51532
• Specificity: Detects human Brg1 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Knockout Validated
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Brg1 Antibody [Alexa Fluor® 488]

• ATP-dependent helicase SMARCA4
• BAF190
• BAF190A
• brahma protein-like 1
• Brg1
• BRG1-associated factor 190A
• BRG1SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 4
• BRM/SWI2-related gene 1
• EC 3.6.1
• EC 3.6.4.-
• FLJ39786
• global transcription activator homologous sequence
• homeotic gene regulator
• hSNF2b
• Mitotic growth and transcription activator
• nuclear protein GRB1
• Protein brahma homolog 1
• Protein BRG-1
• RTPS2
• SMARCA4
• SNF2
• SNF2B
• SNF2-beta
• SNF2L4
• SNF2LB
• SNF2-like 4
• subfamily a, member 4
• sucrose nonfermenting-like 4
• SWI/SNF related, matrix associated, actin dependent regulator of chromatin
• SWI2
• transcription activator BRG1

Background

Brg1 (Brm/Swi2-related gene 1), also known as SMARCA4 (Swi/SNF-related matrix-associated actin-dependent regulator of chromatin, subfamily A, member 4), SNF2L4 and SNF2 beta , is a 205 kDa member of the SNF2 helicase family of molecules. It is a ubiquitously expressed, nuclear-localized chromatin remodeling ATPase that, depending upon its associated complex, may both facilitate and inhibit gene transcription. Human SMARCA4 is 1647 amino acids (aa) in length, and contains an HSA domain (aa 460‑532), one BRK region (aa 610‑654), six potential phosphoserine sites between aa 610‑662, a DEXDc domain that unwinds RNA and DNA (aa 774‑913), one Bromo domain (aa 1477‑1547) and eight potential phosphoserine sites between aa 1570‑1644.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.