BRDG1/STAP-1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: BRDG1/STAP-1 Antibody [NBP1-89941] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.

Immunohistochemistry-Paraffin: BRDG1/STAP-1 Antibody [NBP1-89941] - Staining in human lymph node and cerebral cortex tissues using anti-STAP1 antibody. Corresponding STAP1 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: BRDG1/STAP-1 Antibody [NBP1-89941] - Staining of human lymph node shows high expression.

Immunohistochemistry-Paraffin: BRDG1/STAP-1 Antibody [NBP1-89941] - Staining of human cerebral cortex shows low expression as expected.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

BRDG1/STAP-1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit BRDG1/STAP-1 Antibody - BSA Free (NBP1-89941) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: ESGEEWRGFILTVTELSVPQNVSLLPGQVIKLHEVLEREKKRRIETEQSTSVEKEKEPTEDYVDVLNPMPACFYTVSRKEATEMLQK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: STAP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  BRDG1/STAP-1 Protein (NBP1-89941PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (80%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for BRDG1/STAP-1 Antibody - BSA Free

• BCR downstream-signaling protein 1
• BRDG1
• BRDG1STAP-1BCR downstream signaling 1
• Docking protein BRDG1
• signal transducing adaptor family member 1
• signal-transducing adaptor protein 1
• signal-transducing adaptor protein-1
• STAP1
• Stem cell adaptor protein 1

Background

STAP-1 is encoded by this gene functions as a docking protein acting downstream of Tec tyrosine kinase in B cell antigen receptor signaling. The protein is directly phosphorylated by Tec in vitro where it participates in a postive feedback loop, increasing Tec activity. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STAP1