Home » BRCAA1 » BRCAA1 Antibodies » BRCAA1 Antibody

BRCAA1 Antibody

 (1 Publication)
 (1 Review)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western Blot: BRCAA1 Antibody [NBP1-26618] - Whole cell lysate from HeLa cells. ARID4B was also immunoprecipitated by rabbit anti-ARID4B antibody NBP1-26619.
Western Blot: BRCAA1 Antibody [NBP1-26618] - Whole cell lysate from HeLa cells. ARID4B was also immunoprecipitated by rabbit anti-ARID4B antibody NBP1-26619.
Immunohistochemistry: BRCAA1 Antibody [NBP1-26618] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ARID4B used at a dilution of 1:1,000 ( 1ug/ml). Detection: DAB staining using ...read more
Immunohistochemistry: BRCAA1 Antibody [NBP1-26618] - FFPE section of human ovarian carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunohistochemistry: BRCAA1 Antibody [NBP1-26618] - FFPE section of human ovarian carcinoma. Antibod used at a dilution of 1:100. Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed ...read more
Proximity Ligation Assay: BRCAA1 Antibody [NBP1-26618] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SDS3 (NB100-257) and rabbit anti-human ARID4B (NBP1-26618). Image merged from DAPI (2ms) and ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, IHC, IHC-P, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

100% Guarantee on every product

BRCAA1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 125 and 175 of human AT rich interactive domain 4B using the numbering given in entry NP_057458.4 (GeneID 51742).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ARID4B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Chromatin Immunoprecipitation
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 5-10 ug/mg lysate
  • Proximity Ligation Assay 1:1000-1:20000
Application Notes
Use in immunprecipitation and chromatin immunoprecipitation reported in scientific literature (PMID 25351346)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-26618 in the following applications:
Publications
Read Publication using
NBP1-26618 in the following applications:

  • ChIP
    1 publication
  • IP
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BRCAA1 Antibody

  • 180 kDa Sin3-associated polypeptide
  • ARID domain-containing protein 4B
  • AT rich interactive domain 4B (RBP1- like)
  • AT rich interactive domain 4B (RBP1-like)
  • AT-rich interactive domain-containing protein 4B
  • BRCAA1DKFZp313M2420
  • breast cancer-associated antigen 1
  • Breast cancer-associated antigen BRCAA1
  • breast carcinoma-associated antigen
  • Histone deacetylase complex subunit SAP180
  • Rb-binding protein homolog
  • RBBP1L1RBP1L1BCAA
  • RBP1-like protein
  • retinoblastoma binding protein 1-like 1
  • Retinoblastoma-binding protein 1-like 1
  • SAP180MGC163290
  • SIN3A-associated protein 180
  • Sin3-associated polypeptide p180

Background

ARID4B (AT-rich interactive domain-containing protein 4B) is also known as RbBP1L1 (retinoblastoma binding protein 1-like 1) and is a retinoblastoma binding protein and member of the AT-rich interaction domain (ARID) family of proteins. In addition to the ARID DNA binding domain, ARID4B also contains a chromo domain and a TUDOR domain, indicating a function in chromatin organization and protein-protein interactions with methylated protein substrates. As a subunit of the SIN3A transcriptional co-repressor, ARID4B is implicated to be involved in chromatin remodeling and epigenetic modifications. ARID4B may play a role in the pathogenesis of breast cancer and other malignancies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Insulin Antibody
(E2-E3 (same as INS04 ...
NBP2-34260
Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)
SDS-Page: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260]
(4 Reviews)
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
beta Amyloid
Antibody (MOAB-2)
NBP2-13075
Immunohistochemistry: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - IHC analysis of beta Amyloid on normal mouse brain (left) and 5xFAD mouse brain (right) using DAB with hematoxylin counterstain. The MOAB-2 antibody was used at 1:20 on normal mouse brain and at 1:400 on 5xFAD mouse brain.
Immunohistochemistry-Paraffin: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - IHC analysis of a formalin fixed paraffin embedded tissue section of human brain (Alzheimers disease, hippocampus) using 1:200 dilution of anti-beta Amyloid antibody (clone MOAB-2). The staining was developed with HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This beta Amyloid antibody specifically stained the cells with Abeta 42/ Abeta aggregates while the normal cells were negative for abeta peptide.
Western Blot: beta Amyloid Antibody (MOAB-2) [NBP2-13075] - Western blot analysis in cell lysates from HEK-APP SWE/BACE1 cells probed with an antibody against the C-terminus of APP (Lanes 1 and 2) and beta Amyloid (MOAB-2, Lanes 3 and 4). Beta Amyloid (MOAB-2) does not detect APP (from PMID: 22423893).
(12 Publications)
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, IHC-FrFl
Albumin Antibody
NB600-41532
Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.
(38 Publications)
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
AAAS Antibody (5A1)
H00008086-M02
Western Blot: AAAS Antibody (5A1) [H00008086-M02] - AAAS monoclonal antibody (M02), clone 5A1. Analysis of AAAS expression in NIH/3T3.
Immunohistochemistry-Paraffin: AAAS Antibody (5A1) [H00008086-M02] - Analysis of monoclonal antibody to AAAS on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml.
Western Blot: AAAS Antibody (5A1) [H00008086-M02] - Western Blot analysis of AAAS expression in HeLa ( Cat # L013V1 ).
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC-P
TEF1 Antibody
(13112)
NBP2-43581
Western Blot: TEF1 Antibody (13112) [NBP2-43581] - Analysis of whole cell extracts and extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TEF1 antibody [13112] at a dilution of 1:500.
Immunocytochemistry/Immunofluorescence: TEF1 Antibody (13112) [NBP2-43581] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TEF1 protein stained by TEF1 antibody [GT13112] diluted at 1:400. Red: Phalloidin, a F-actin marker, diluted at 1:200.
Immunoprecipitation: TEF1 Antibody (13112) [NBP2-43581] - Immunoprecipitation of TEF1 protein from HeLa nuclear extracts using 5 ug of TEF1 antibody [13112]. Western blot analysis was performed using TEF1 antibody [13112] EasyBlot anti-Mouse IgG was used as a secondary reagent.
Species: Hu
Applications: WB, ICC/IF, IP
TOR/mTOR [p
Ser2448] Antibody
NB600-607
Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Detection of a 250 kDa band (indicated) corresponding to phosphorylated human mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. 20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Used at 5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate staining of proximal convoluted tubules of the kidney. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
(5 Publications)
Species: Hu, Rt
Applications: WB, EM, ELISA, IHC, IHC-P
Ribosomal Protein
S6/RPS6 Antibody
NB100-1595
Western Blot: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Detection of Human RPS6 on HeLa whole cell lysate using NB100-1595. RPS6 was also immunoprecipitated using rabbit anti-RPS6 antibodies NB100-1594 and another RPS6 Ab.
Immunohistochemistry: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti- RPS6 used at a dilution of 1:200 (1ug/ml). Detection: DAB
(1 Review)
(2 Publications)
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
TNF-alpha
[Unconjugated]
210-TA/CF
Recombinant Human TNF-alpha (Catalog # 210‑TA/CF) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
(475 Publications)
Species: Hu
Tyrosinase
Antibody (T311)
NBP2-25238
Immunohistochemistry-Paraffin: Tyrosinase Antibody (T311) [NBP2-25238] - Formalin-paraffin melanoma (20X) stained with Tyrosinase Ab (T311).
Immunohistochemistry-Paraffin: Tyrosinase Antibody (T311) [NBP2-25238] - Formalin-paraffin melanoma (10X) stained with Tyrosinase Ab (T311).
SDS-Page: Tyrosinase Antibody (T311) [NBP2-25238]
Species: Hu
Applications: Flow, ICC/IF, IHC-P, PAGE
4EBP1 Antibody
NB200-157
Western Blot: 4EBP1 Antibody [NB200-157] - Whole cell lysate (100 ug) from RINm5F insulinoma exposed to high or low concentration of leucine. Antibody used at 0.25 ug/ml.
Immunohistochemistry: 4EBP1 Antibody [NB200-157] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-4EBP1 used at a dilution of 1:20,000 ( 0.05ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.
(45 Publications)
Species: Hu
Applications: WB, IHC, IHC-P
Glucagon Antibody
(181402) [Unconjugate ...
MAB1249
Glucagon was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using 10 µg/mL Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
beta TC‑6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse Glucagon Monoclonal Antibody (Catalog # MAB1249, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
(3 Reviews)
(3 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, ICC, ICFlow
Prolactin Antibody
[Unconjugated]
AF682
Prolactin was detected in immersion fixed paraffin-embedded sections of human testis using Goat Anti-Human Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF682) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of sperm cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western blot shows lysates of human pituitary tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF682) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Prolactin at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Recombinant Human Prolactin (Catalog # 682-PL) stimulates proliferation in the Nb2‑11 rat lymphoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Prolactin (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Prolactin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF682). The ND50 is typically 0.02-0.05 µg/mL.
(3 Publications)
Species: Hu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
TYRP1 Antibody
NBP1-88370
Western Blot: TYRP1 Antibody [NBP1-88370] - Analysis in human cell line SK-MEL-30.
Immunocytochemistry/Immunofluorescence: TYRP1 Antibody [NBP1-88370] - Immunofluorescent staining of human cell line SK-MEL-30 shows localization to vesicles.
Immunohistochemistry-Paraffin: TYRP1 Antibody [NBP1-88370] - Staining in human skin and pancreas tissues using anti-TYRP1 antibody. Corresponding TYRP1 RNA-seq data are presented for the same tissues.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
TBP like protein
TLP Antibody
NBP1-31133
Western Blot: TBP like protein TLP Antibody [NBP1-31133] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:2000.
Immunocytochemistry/Immunofluorescence: TBP like protein TLP Antibody [NBP1-31133] - NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TLP protein stained by TLP antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: TBP like protein TLP Antibody [NBP1-31133] - Paraffin-embedded mouse lung. TLP antibody dilution: 1:500.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
BCAT2 Antibody
NBP2-33705
Western Blot: BCAT2 Antibody [NBP2-33705] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4
Immunohistochemistry-Paraffin: BCAT2 Antibody [NBP2-33705] - Staining in human adrenal gland and liver tissues using anti-BCAT2 antibody. Corresponding BCAT2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: BCAT2 Antibody [NBP2-33705] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Species: Hu
Applications: WB, IHC, IHC-P
Transferrin
Antibody (HTF-14)
NB500-418
Western Blot: Transferrin Antibody (HTF-14) [NB500-418] - Analysis using the Azide Free version of NB500-418. Detection of 1. hTransferrin; 5 ug/well (red. con.) 2. hTransferrin; 3 ug/well (red. con.) 3. hTransferrin; 1 ug/well (red. con.) M. Low Range marker 4. hTransferrin; 1 ug/well (non-red. con.) 5. hTransferrin; 3 ug/well (non-red. con.) 6. hTransferrin; 5 ug/well (non-red. con.)
(1 Review)
Species: Hu, Po, Rb
Applications: WB, B/N, ELISA, ICC/IF, IHC, IHC-P, RIA
Tbp7 Antibody
NBP1-87799
Western Blot: Tbp7 Antibody [NBP1-87799] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.
Immunocytochemistry/Immunofluorescence: Tbp7 Antibody [NBP1-87799] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: Tbp7 Antibody [NBP1-87799] - Staining of human gall bladder shows nuclear positivity in glandular cells.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
IGF-I
[Unconjugated]
291-G1
1 μg/lane of Recombinant Human IGF-I was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.
Recombinant Human IGF-I (Catalog # 291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line. The ED50 for this effect is 0.3‑1.5 ng/mL.
MALDI-TOF analysis of Recombinant Human IGF-I.The major peak corresponds to the calculated molecular mass, 7650 Da. The minor peak at 7873 is a matrix-associated artifact of the MALDI-TOF.
(2 Reviews)
(92 Publications)
Species: Hu
Retinoblastoma
binding protein 1 Ant ...
NBP1-26616
Western Blot: Retinoblastoma binding protein 1 Antibody [NBP1-26616] - Whole cell lysate from HeLa cells. ARID4A was also immunoprecipitated by rabbit anti-ARID4A antibody NBP1-26617.
Species: Hu
Applications: WB, IP
BRCAA1 Antibody
NBP1-26618
BRCAA1 Antibody
Species: Hu
Applications: WB, ChIP, IHC, IHC-P, IP, PLA

Publications for BRCAA1 Antibody (NBP1-26618)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ChIP, IP, WB.


Filter By Application
ChIP
(1)
IP
(1)
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

Review for BRCAA1 Antibody (NBP1-26618) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-26618:
Filter by Applications
IP
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
rajesha rupaimoole
 IP Human 10/06/2014
View

Summary

ApplicationImmunoprecipitation
Sample TestedHuman cancer cell line A2780, MCF7
SpeciesHuman

Blocking

Blocking DetailsStandard according to protocol

Primary Anitbody

Dilution Ratio5ul of antibody per 300ul IP mix

Details

Immunoprecipitation DetailsAgarose G magnetic beads, Used kit from Active Motif - Universal Magnetic Co-IP Kit
Elution DescriptionStandard according to protocol

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol

FAQs for BRCAA1 Antibody (NBP1-26618) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional BRCAA1 Products

Bioinformatics Tool for BRCAA1 Antibody (NBP1-26618)

Discover related pathways, diseases and genes to BRCAA1 Antibody (NBP1-26618). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for BRCAA1 Antibody (NBP1-26618)

Discover more about diseases related to BRCAA1 Antibody (NBP1-26618).
 

Pathways for BRCAA1 Antibody (NBP1-26618)

View related products by pathway.

PTMs for BRCAA1 Antibody (NBP1-26618)

Learn more about PTMs related to BRCAA1 Antibody (NBP1-26618).
 

Research Areas for BRCAA1 Antibody (NBP1-26618)

Find related products by research area.

Blogs on BRCAA1

There are no specific blogs for BRCAA1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0
 
 rajesha rupaimoole
10/06/2014
Application: IP
Species: Human
read this review

Bioinformatics

Gene Symbol ARID4B
Entrez
Uniprot