Bovine Fibronectin Protein, CF

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Product Details

Summary
Reactivity BvSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Bovine Fibronectin Protein, CF Summary

Details of Functionality
Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. In this application, the recommended concentration for this effect is
1‑5 µg/cm2.  Fibronectin can also be added in the media to support cell spreading at a concentration of 0.5-50 µg/mL.
Optimal concentration depends on cell type as well as the application or research objectives.
Source
Bovine plasma-derived Fibronectin protein
Protein/Peptide Type
Natural Proteins
Gene
FN1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Publications
Read Publications using
1030-FN in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 6 months, 2 to 8 °C under sterile conditions after opening.
Avoid vortexing and excessive agitation.
Buffer
Supplied as a 0.2 μm filtered solution in Tris-HCl, NaCl and Urea.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Bovine Fibronectin Protein, CF

  • CIG
  • ED-B
  • fibronectin 1
  • Fibronectin
  • FINC
  • FN
  • FN1
  • FNZ
  • GFND
  • GFND2
  • LETS
  • MSF
  • SMDCF

Background

Fibronectin is an extracellular matrix (ECM) component and is one of the primary cell adhesion molecules (1). It is composed of multiple homologous repeats and contains many functional domains. The occurrence of different isoforms is due to alternative mRNA splicing of the ED-A, ED-B and III-CS regions and subsequent post-translational modification. Although non-reactive with adhesion receptors in its soluble state, Fibronectin is highly adhesive when on the surface (2). Polymerization of Fibronectin into ECM must be tightly regulated to ensure appropriate adhesive proporties upon ECM formation. Because of its ability to interact with many ligands (e.g. cells, heparin, fibrin, collagen, DNA, immunoglobulin), Fibronectin plays an important role in normal morphogenesis, including cell adhesion, migration, differentiation, and specific gene expression (3 - 6).

  1. Vaheri, A. et al. (1978) Biochem. Biophys. Acta. 516:1.
  2. Pytela, R. et al. (1985) Proc. Natl. Acad. Sci. USA 82:5766.
  3. Danen, E.H. et al. (2001) J. Cell. Physiol. 189:1.
  4. Pereira, M. et al. (2001) Am. NY Acad. Sci. 936:438.
  5. Ruoslahti, E. (1999) Adv. Cancer Review 76:1.
  6. Romberger, D.J. (1997) Int. J. Biochem. Cell Biol. 29:939.

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1030-FN
Species: Bv
Applications: Bioactivity

Publications for Fibronectin (1030-FN)(9)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Hamster, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 2 applications: Adhesion Assay, Bioassay.


Filter By Application
Adhesion Assay
(1)
Bioassay
(8)
All Applications
Filter By Species
Human
(2)
Mouse
(4)
Rat
(1)
Hamster
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using 1030-FN Applications Species
N Boukelmoun, GS Chiu, A Kavelaars, CJ Heijnen Mitochondrial transfer from mesenchymal stem cells to neural stem cells protects against the neurotoxic effects of cisplatin Acta Neuropathol Commun, 2018;6(1):139. 2018 [PMID: 30541620] (Bioassay, Mouse) Bioassay Mouse
SU Vay, LJ Flitsch, M Rabenstein, R Rogall, S Blaschke, J Kleinhaus, N Reinert, A Bach, GR Fink, M Schroeter, MA Rueger The plasticity of primary microglia and their multifaceted effects on endogenous neural stem cells in vitro and in vivo J Neuroinflammation, 2018;15(1):226. 2018 [PMID: 30103769] (Bioassay, Rat) Bioassay Rat
J Yang, C Wu, I Stefanescu, A Horowitz Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies J Vis Exp, 2017;0(122):. 2017 [PMID: 28518115] (Bioassay, Mouse) Bioassay Mouse
Adepoju A, Micali N, Ogawa K, Hoeppner D, McKay R FGF2 and insulin signaling converge to regulate cyclin D expression in multipotent neural stem cells. Stem Cells, 2014;32(3):770-8. 2014 [PMID: 24155149] (Bioassay, Mouse) Bioassay Mouse
Li YY, Bao YL, Song ZB, Sun LG, Wu P, Zhang Y, Fan C, Huang YX, Wu Y, Yu CL, Sun Y, Zheng LH, Wang GN, Li YX The threonine protease activity of testes-specific protease 50 (TSP50) is essential for its function in cell proliferation. PLoS ONE, 2012;7(5):e35030. 2012 [PMID: 22574111] (Adhesion Assay, Hamster) Adhesion Assay Hamster
Sasaki K, Pardee AD, Okada H, Storkus WJ IL-4 inhibits VLA-4 expression on Tc1 cells resulting in poor tumor infiltration and reduced therapy benefit. Eur. J. Immunol., 2008;38(10):2865-73. 2008 [PMID: 18958887] (Bioassay, Mouse) Bioassay Mouse
Isakova IA, Baker K, Dutreil M, Dufour J, Gaupp D, Phinney DG Age- and dose-related effects on MSC engraftment levels and anatomical distribution in the central nervous systems of nonhuman primates: identification of novel MSC subpopulations that respond to guidance cues in brain. Stem Cells, 2007;25(12):3261-70. 2007 [PMID: 17932418] (Bioassay, Primate - Macaca mulatta (Rhesus Macaque)) Bioassay Primate - Macaca mulatta (Rhesus Macaque)
Shintani Y, Maeda M, Chaika N, Johnson KR, Wheelock MJ Collagen I promotes epithelial-to-mesenchymal transition in lung cancer cells via transforming growth factor-beta signaling. Am. J. Respir. Cell Mol. Biol., 2007;38(1):95-104. 2007 [PMID: 17673689] (Bioassay, Human) Bioassay Human
Ni HT, Hu S, Sheng WS, Olson JM, Cheeran MC, Chan AS, Lokensgard JR, Peterson PK High-level expression of functional chemokine receptor CXCR4 on human neural precursor cells. Brain Res. Dev. Brain Res., 2004;152(2):159-69. 2004 [PMID: 15351504] (Bioassay, Human) Bioassay Human

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Nickel induces migratory and invasive phenotype in human epithelial cells by epigenetically activating ZEB1
By Jamshed Arslan Pharm.D. Nickel (Ni) is a naturally abundant metallic element. It is a major component of stainless steel, coins, and many other items of daily use. Disturbingly, Ni exposure is associated with can...  Read full blog post.

Bad news for stomach cancer: BAMBI protein inhibits gastric carcinoma via TGF-beta/epithelial-mesenchymal transition signaling
By Jamshed Arslan Pharm.D. Gastric carcinoma is the second leading cause of cancer-related deaths worldwide. One of the key features of gastric carcinoma is acidosis, which promotes growth and metastasis of gastric...  Read full blog post.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Fibronectin: Organizing Cell Activity across the ECM
Fibronectin is a glycoprotein found in the extracellular matrix (ECM) that binds to integrins and other components of the ECM such as collagen and fibrin. Under normal physiological conditions, fibronectin is an important factor in cell adhesion, grow...  Read full blog post.

Using the Laminin Antibody in Angiogenesis Research
Laminin is one of a large number of proteins expressed on the basal laminar of the ECM (extracellular matrix). The laminin antibody database covers several proteins, which interact with integrins and other receptor proteins to support cellular differe...  Read full blog post.

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Bioinformatics

Gene Symbol FN1