Bovine Fibronectin Protein, CF

Dopaminergic neurons were generated from human pluripotent stem cells in media that included Bovine Fibronectin Protein (Catalog # 1030-FN) to support cell attachment and spreading, the ITS and N-2 Plus Media Supplements (AR013 and AR003, respectively) to select and enrich for neural stem cell populations, and a panel of growth factors for effective dopaminergic differentiation, including Recombinant Human FGF-basic, Recombinant Mouse FGF-8b (423-F8), and Recombinant Mouse Shh-N (464-SH). Cells were stained with a Mouse Anti-Human/Mouse Tyrosine Hydroxylase Monoclonal Antibody (MAB7566) followed by a NorthernLights™ 493-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (NL009; green), and a Mouse Neuron-specific beta III Tubulin Tuj1 Monoclonal Antibody (MAB1195) followed by a NorthernLights 557-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (NL007; red) and counterstained with DAPI (5748; blue).

Dopaminergic neurons were generated from human pluripotent stem cells in media that included Bovine Fibronectin Protein (Catalog # 1030-FN) to support cell attachment and spreading, the ITS and N-2 Plus Media Supplements (AR013 and AR003, respectively), and a panel of growth factors for effective dopaminergic differentiation, including Recombinant Human FGF-basic, Recombinant Mouse FGF-8b (423-F8), and Recombinant Mouse Shh-N (464-SH). Tyrosine Hydroxylase was detected using a Mouse Anti-Human Tyrosine Hydroxylase Monoclonal Antibody (MAB7566). The cells were stained with the NorthernLights™ 557-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (NL007; red). Neuron-specific beta-III Tubulin was detected using a Mouse Anti-Neuron-specific beta-III Tubulin (Clone Tuj-1) Monoclonal Antibody (MAB1195) followed by the NorthernLights™ 493-conjugated Donkey Anti-Mouse IgG Antigen Affinity-purified Secondary Antibody (NL009; green). Cells were counterstained with DAPI (5748; blue).

D3 mouse embryonic stem cells were expanded in KO-ES Media supplemented with Bovine Fibronectin Protein (Catalog # 1030-FN) to support cell attachment and spreading, the ITS and N-2 Plus Media Supplements (AR013 and AR003), and a panel of growth factors for effective oligodendrocyte differentiation, including Recombinant Human FGF-basic, Recombinant Human EGF (236-EG), and Recombinant Human PDGF-AA (221-AA). Oligodendrocytes were detected using a Mouse Anti-Human/Mouse/Rat/Chicken Oligodendrocyte Marker O4 Monoclonal Antibody (MAB1326). The cells were stained with the NorthernLights™-557 Affinity-purified Goat Anti-Mouse IgM Secondary Antibody (NL019; red). The nuclei were counterstained with DAPI (5748;blue).

• Reactivity: Bv
• Applications: Bioactivity
• Format: Carrier-Free

Bovine Fibronectin Protein, CF Summary

• Details of Functionality: Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. In this application, the recommended concentration for this effect is
  1‑5 µg/cm². Fibronectin can also be added in the media to support cell spreading at a concentration of 0.5-50 µg/mL.
  Optimal concentration depends on cell type as well as the application or research objectives. Measured by the ability of the immobilized protein to support the adhesion of B16‑F1 mouse melanoma cells. The ED₅₀ for this effect is 10.0-100 ng/mL.
• Source: Bovine plasma-derived Fibronectin protein
• Protein/Peptide Type: Natural Proteins
• Gene: FN1
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Bioactivity2

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  Thaw frozen material overnight at 2-8 °C. Do not disturb the product during the initial thaw event. Avoid vortexing and excessive agitation of the product.
• Buffer: Supplied as a 0.2 μm filtered solution in Tris-HCl, NaCl and Urea.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Bovine Fibronectin Protein, CF

• CIG
• ED-B
• fibronectin 1
• Fibronectin
• FINC
• FN
• FN1
• FNZ
• GFND
• GFND2
• LETS
• MSF
• SMDCF

Background

Fibronectin is an extracellular matrix (ECM) component and is one of the primary cell adhesion molecules (1). It is composed of multiple homologous repeats and contains many functional domains. The occurrence of different isoforms is due to alternative mRNA splicing of the ED-A, ED-B and III-CS regions and subsequent post-translational modification. Although non-reactive with adhesion receptors in its soluble state, Fibronectin is highly adhesive when on the surface (2). Polymerization of Fibronectin into ECM must be tightly regulated to ensure appropriate adhesive proporties upon ECM formation. Because of its ability to interact with many ligands (e.g. cells, heparin, fibrin, collagen, DNA, immunoglobulin), Fibronectin plays an important role in normal morphogenesis, including cell adhesion, migration, differentiation, and specific gene expression (3 - 6).

1. Vaheri, A. et al. (1978) Biochem. Biophys. Acta. 516:1.
2. Pytela, R. et al. (1985) Proc. Natl. Acad. Sci. USA 82:5766.
3. Danen, E.H. et al. (2001) J. Cell. Physiol. 189:1.
4. Pereira, M. et al. (2001) Am. NY Acad. Sci. 936:438.
5. Ruoslahti, E. (1999) Adv. Cancer Review 76:1.
6. Romberger, D.J. (1997) Int. J. Biochem. Cell Biol. 29:939.

Publications for Fibronectin (1030-FN)(17)

Publications using 1030-FN

• Song, X;Wu, W;Varshney, M;Roman, A;Gustafsson, JÅ;Warner, M; Role of LXR? in oligodendrocytes in neuronal survival Molecular psychiatry 2025-10-01 [PMID: 41034505] (Bioassay, Mouse)
• Mato-Blanco, X;Kim, SK;Jourdon, A;Ma, S;Choi, SH;Giani, AM;Paredes, MI;Tebbenkamp, ATN;Liu, F;Duque, A;Vaccarino, FM;Sestan, N;Colantuoni, C;Rakic, P;Santpere, G;Micali, N; Early developmental origins of cortical disorders modeled in human neural stem cells Nature communications 2025-07-09 [PMID: 40634286] (Bioassay, Human)
• Frey, N;Ouologuem, L;Blenninger, J;Siow, WX;Thorn-Seshold, J;Stöckl, J;Abrahamian, C;Fröhlich, T;Vollmar, AM;Grimm, C;Bartel, K; Endolysosomal TRPML1 channel regulates cancer cell migration by altering intracellular trafficking of E-cadherin and ?1-integrin The Journal of biological chemistry 2023-12-21 [PMID: 38141765] (Bioassay, Human, Mouse)
• Zheng, C;Wei, Y;Zhang, Q;Sun, M;Wang, Y;Hou, J;Zhang, P;Lv, X;Su, D;Jiang, Y;Gumin, J;Sahni, N;Hu, B;Wang, W;Chen, X;McGrail, DJ;Zhang, C;Huang, S;Xu, H;Chen, J;Lang, FF;Hu, J;Chen, Y; Multiomics analyses reveal DARS1-AS1/YBX1-controlled posttranscriptional circuits promoting glioblastoma tumorigenesis/radioresistance Science advances 2023-08-04 [PMID: 37540752] (Bioassay, Human)
• M Tobiume, A Mitsuhashi, A Saijo, H Ogino, T Afroj, H Ogawa, H Goto, S Sato, A Abe, K Haji, R Ozaki, H Takizawa, Y Nishioka Analysis of the chemotactic factors for tumor-infiltrating fibrocytes and their prognostic significances in lung cancer Oncology Letters, 2022-09-30;24(5):417. 2022-09-30 [PMID: 36245829] (Bioassay, Human)
• P Marchese, M Lombardi, ME Mantione, D Baccellier, D Ferrara, R Chiesa, O Alfieri, C Foglieni Confocal Blood Flow Videomicroscopy of Thrombus Formation over Human Arteries and Local Targeting of P2X7 International Journal of Molecular Sciences, 2021-04-14;22(8):. 2021-04-14 [PMID: 33920051] (Bioassay, Human)
• N Micali, SK Kim, M Diaz-Busta, G Stein-O'Br, S Seo, JH Shin, BG Rash, S Ma, Y Wang, NA Olivares, JI Arellano, KR Maynard, EJ Fertig, AJ Cross, RW Bürli, NJ Brandon, DR Weinberger, JG Chenoweth, DJ Hoeppner, N Sestan, P Rakic, C Colantuoni, RD McKay Variation of Human Neural Stem Cells Generating Organizer States In�Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates Cell Rep, 2020-05-05;31(5):107599. 2020-05-05 [PMID: 32375049] (Cell Culture, Mouse)
• SY Ahn, YS Maeng, YR Kim, YH Choe, HS Hwang, YM Hyun In vivo monitoring of dynamic interaction between neutrophil and human umbilical cord blood-derived mesenchymal stem cell in mouse liver during sepsis Stem Cell Res Ther, 2020-02-03;11(1):44. 2020-02-03 [PMID: 32014040] (Bioassay, Human)
• N Boukelmoun, GS Chiu, A Kavelaars, CJ Heijnen Mitochondrial transfer from mesenchymal stem cells to neural stem cells protects against the neurotoxic effects of cisplatin Acta Neuropathol Commun, 2018-12-12;6(1):139. 2018-12-12 [PMID: 30541620] (Bioassay, Mouse)
• SU Vay, LJ Flitsch, M Rabenstein, R Rogall, S Blaschke, J Kleinhaus, N Reinert, A Bach, GR Fink, M Schroeter, MA Rueger The plasticity of primary microglia and their multifaceted effects on endogenous neural stem cells in vitro and in vivo J Neuroinflammation, 2018-08-13;15(1):226. 2018-08-13 [PMID: 30103769] (Bioassay, Rat)
• J Yang, C Wu, I Stefanescu, A Horowitz Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies J Vis Exp, 2017-04-22;0(122):. 2017-04-22 [PMID: 28518115] (Bioassay, Mouse)
• Adepoju A, Micali N, Ogawa K, Hoeppner D, McKay R FGF2 and insulin signaling converge to regulate cyclin D expression in multipotent neural stem cells. Stem Cells, 2014-03-01;32(3):770-8. 2014-03-01 [PMID: 24155149] (Bioassay, Mouse)
• Li YY, Bao YL, Song ZB, Sun LG, Wu P, Zhang Y, Fan C, Huang YX, Wu Y, Yu CL, Sun Y, Zheng LH, Wang GN, Li YX The threonine protease activity of testes-specific protease 50 (TSP50) is essential for its function in cell proliferation. PLoS ONE, 2012-05-04;7(5):e35030. 2012-05-04 [PMID: 22574111] (Adhesion Assay, Hamster)
• Sasaki K, Pardee AD, Okada H, Storkus WJ IL-4 inhibits VLA-4 expression on Tc1 cells resulting in poor tumor infiltration and reduced therapy benefit. Eur. J. Immunol., 2008-10-01;38(10):2865-73. 2008-10-01 [PMID: 18958887] (Bioassay, Mouse)
• Isakova IA, Baker K, Dutreil M, Dufour J, Gaupp D, Phinney DG Age- and dose-related effects on MSC engraftment levels and anatomical distribution in the central nervous systems of nonhuman primates: identification of novel MSC subpopulations that respond to guidance cues in brain. Stem Cells, 2007-10-11;25(12):3261-70. 2007-10-11 [PMID: 17932418] (Bioassay, Primate - Macaca mulatta (Rhesus Macaque))
• Shintani Y, Maeda M, Chaika N, Johnson KR, Wheelock MJ Collagen I promotes epithelial-to-mesenchymal transition in lung cancer cells via transforming growth factor-beta signaling. Am. J. Respir. Cell Mol. Biol., 2007-08-02;38(1):95-104. 2007-08-02 [PMID: 17673689] (Bioassay, Human)
• Ni HT, Hu S, Sheng WS, Olson JM, Cheeran MC, Chan AS, Lokensgard JR, Peterson PK High-level expression of functional chemokine receptor CXCR4 on human neural precursor cells. Brain Res. Dev. Brain Res., 2004-09-17;152(2):159-69. 2004-09-17 [PMID: 15351504] (Bioassay, Human)

Bioinformatics

• Gene Symbol: FN1