BOC Antibody [Unconjugated]

BOC was detected in perfusion fixed frozen sections of mouse embryo using Goat Anti-Mouse BOC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2385) at 15 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004) or the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of the neural tube, spinal cord. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

BOC Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse BOC
  Asp26-Lys508 (Gln ins) Val509-Asp846
  Accession # AAH78631
• Specificity: Detects mouse BOC in direct ELISAs and Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant human BOC is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BOC
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BOC Antibody [Unconjugated]

• Boc homolog (mouse)
• BOC
• brother of CDO
• Protein BOC

Background

BOC and CDO (cell adhesion molecule-related/down-regulated by oncogenes) are cell surface receptors belonging to the Ig/fibronectin type III (FNIII) repeat family. Both proteins have five Ig repeats and three FNIII repeats in their extracellular domains, an arrangement shared by Robo proteins. BOC and CDO are co-expressed in muscle precursor cells during development. BOC associates with CDO in cis and forms complexes with cadherins to regulate myogenic differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BOC Antibody (AF2385)(6)

Publications using AF2385

• Krueger, MR;Cheema, SK;Simo, S;Levine, EM;Brown, NL;La Torre, A; Novel Roles of Sonic Hedgehog Signaling in Retinal Patterning and Neurogenesis During Mammalian Eye Development bioRxiv : the preprint server for biology 2025-08-21 [PMID: 40894764] (Immunohistochemistry, Mouse, Transgenic Mouse)
• Lee, J;Kim, Y;Ataliotis, P;Kim, HG;Kim, DW;Bennett, DC;Brown, NA;Layman, LC;Kim, SH; Coordination of canonical and noncanonical Hedgehog signalling pathways mediated by WDR11 during primordial germ cell development Scientific reports 2023-07-29 [PMID: 37516749] (Western Blot, Mouse)
• Y Kim, J Lee, M Seppala, MT Cobourne, SH Kim Ptch2/Gas1 and Ptch1/Boc differentially regulate Hedgehog signalling in murine primordial germ cell migration Nat Commun, 2020-04-24;11(1):1994. 2020-04-24 [PMID: 32332736] (IHC, Mouse)
• TA Vuong, SJ Lee, YE Leem, JR Lee, GU Bae, JS Kang SGTb regulates a surface localization of a guidance receptor BOC to promote neurite outgrowth Cell. Signal., 2019-01-09;55(0):100-108. 2019-01-09 [PMID: 30639199] (ICC, Mouse)
• Jane Y. Song, Alexander M. Holtz, Justine M. Pinskey, Benjamin L. Allen Distinct structural requirements for CDON and BOC in the promotion of Hedgehog signaling Developmental Biology 2015-06-15 [PMID: 25848697]
• Fabre PJ, Shimogori T, Charron F Segregation of ipsilateral retinal ganglion cell axons at the optic chiasm requires the Shh receptor Boc. J. Neurosci., 2010-01-06;30(0):266. 2010-01-06 [PMID: 20053908] (IHC-P, Mouse, Rat)

Bioinformatics

• Gene Symbol: BOC
• Uniprot:
  • AAH78631()