VC004) or the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog #
HAF109). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog #
VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of the neural tube, spinal cord. View our protocol for
IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

VC004) or the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog #
HAF109). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog #
VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of the neural tube, spinal cord. View our protocol for
IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="BOC was detected in perfusion fixed frozen sections of mouse embryo using Goat Anti-Mouse BOC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2385) at 15 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog #
VC004) or the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog #
HAF109). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog #
VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of the neural tube, spinal cord. View our protocol for
IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />