BNIP3 Antibody [Alexa Fluor® 647]

Product Discontinued

BNIP3 Antibody [Alexa Fluor® 647] Summary

• Immunogen: A synthetic peptide against an N-terminal region (within residues 1-50) of the human BNIP3 protein. [UniProt# Q12983]
• Localization: Mitochondrion. Note: Co-expression with the EIB 19-kDa protein results in a shift in NIP3 localization to the nuclear envelope.
• Predicted Species: Feline (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BNIP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: This BNIP3 antibody is useful for Immunocytochemistry/immunofluorescence and Western Blot. In Western blot a band is seen in hypoxic samples at approx. 30 and 60 kDa, representing the BNIP3 protein. There are some non-specific bands present as well. It is recommended that normoxic samples are run next to the hypoxic samples as a negative control.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human. Immunogen sequence has 93% homology to feline and 87% homology to bovine and canine proteins. Does not work in mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for BNIP3 Antibody [Alexa Fluor® 647]

• BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
• BCL2/adenovirus E1B 19kDa interacting protein 3
• BCL2/adenovirus E1B 19kD-interacting protein 3
• BNIP3
• Nip3

Background

BCL2/adenovirus E1B 19kD protein-interacting protein 3 (BNIP3) is a mitochondrial protein critical for apoptotic events in response to adenovirus infection. It has been implicated in causing a necrotic-like cell death pattern in cells, induced by CD47. This effect has been seen in many cancer cells, and its mis-regulation causes tumor cells to be impervious to normal autophagic events. The tumor suppressor p53 represses the expression of BNIP3, often as a consequence of hypoxic micro-environments as seen in tumors. When BNIP3 is correctly expressed and activated, it translocates to the mitochondria and instigates apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BNIP3