BNIP3 Antibody [Alexa Fluor® 405]

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Product Details

Summary
Reactivity Hu, Mu(-), FelineSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit

BNIP3 Antibody [Alexa Fluor® 405] Summary

Immunogen
A synthetic peptide against an N-terminal region (within residues 1-50) of the human BNIP3 protein. [UniProt# Q12983]
Localization
Mitochondrion. Note: Co-expression with the EIB 19-kDa protein results in a shift in NIP3 localization to the nuclear envelope.
Predicted Species
Feline (93%). Backed by our 100% Guarantee.
Clonality
Polyclonal
Host
Rabbit
Gene
BNIP3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
Alexa Fluor 405
Format
Alexa Fluor 405 (A=405, E=421)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
This BNIP3 antibody is useful for Immunocytochemistry/immunofluorescence and Western Blot. In Western blot a band is seen in hypoxic samples at approx. 30 and 60 kDa, representing the BNIP3 protein. There are some non-specific bands present as well. It is recommended that normoxic samples are run next to the hypoxic samples as a negative control.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human. Immunogen sequence has 93% homology to feline and 87% homology to bovine and canine proteins. Does not work in mouse.

Alternate Names for BNIP3 Antibody [Alexa Fluor® 405]

  • BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
  • BCL2/adenovirus E1B 19kDa interacting protein 3
  • BCL2/adenovirus E1B 19kD-interacting protein 3
  • BNIP3
  • Nip3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for BNIP3 Antibody (NB110-60562AF405) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 488 Labeled NB110-60562AF488
Alexa Fluor 647 Labeled NB110-60562AF647
Alexa Fluor 700 Labeled NB110-60562AF700

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Blogs on BNIP3.

BNIP3 - a regulator of mitochondrial autophagy and cell death
Bcl-2 nineteen-kilodalton interacting protein 3 (BNIP3) is a pro-apoptotic BH3-only protein. BNIP3 localizes to the mitochondrial membrane where it plays a key role in mitochondrial autophagy and cell death pathways. Similar to other Bcl-2 family m...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol BNIP3
Entrez
Uniprot

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