BMP-8 Antibody

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BMP‑8 was detected in immersion fixed paraffin-embedded sections of human osteosarcoma using 15 µg/mL Goat Anti-Human BMP‑8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1073) overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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BMP-8 Antibody Summary

Immunogen
E. coli-derived recombinant human BMP‑8a
Ala264-His402
Accession # AAP74559
Specificity
Detects human BMP-8 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) BMP‑2, rhBMP-3, rhBMP-4, rhBMP-5, rhBMP-6 and rhBMP-7 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
BMP8A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Publications
Read Publication using
AF1073 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BMP-8 Antibody

  • BMP8
  • BMP-8
  • BMP8A
  • BMP-8A
  • OP-2

Background

BMP-8, also known as osteogenic protein 2 (OP-2), was first isolated from a hippocampal library in a screen to identify relatives of BMP-7 (OP-1) (1). BMPs are a family of structurally and functionally related proteins and represent a subfamily of the transforming growth factor beta (TGF-beta ) superfamily. BMPs were originally identified as protein regulators of cartilage and bone formation. They have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs (2). BMPs play roles in regulating growth, differentiation, chemotaxis, and apoptosis of various cell types, including mesenchymal, epithelial, hematopoetic, and neuronal cells.

There exist two highly related and closely linked genes, designated BMP-8a and -8b in mice and humans. For humans, the protein products of these two genes share 98% amino acid (aa) sequence identity in their pro- and mature regions. However in the mouse, the two proteins share 89% and 76% aa sequence homology in their pro- and mature regions, respectively (3). Mature human BMP-8a shares 91% and 70% aa sequence identity with mouse BMP-8a and -8b, respectively. Human BMP‑8a is synthesized as a large precursor protein that is cleaved at a dibasic cleavage site (RTPR) between aa residues 263 and 264 to release a 139 aa carboxy-terminal domain. Expression patterns of the BMP-8 genes indicate that they regulate aspects of cell proliferation and/or differentiation during spermatogenesis and formation of the placenta (3). BMP-8 is also highly expressed in osteosarcomas (4).

  1. Ozkaynak, E. et al. (1992) J. Biol. Chem. 267:25220.
  2. Canalis, E. et al. (2003) Endocrine Rev. 24:218.
  3. Zhao, G-Q. et al. (1996) Mech. Dev. 57:159.
  4. Sulzbacher, I. et al. (2002) J. Clin. Pathol. 55:381.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BMP-8 Antibody (AF1073)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Bioinformatics

Gene Symbol BMP8A
Uniprot