Recombinant Human GDF-7 Protein, CF

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Recombinant Human GDF-7 stimulates alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.25-1.25 μg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human GDF-7 Protein, CF Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is typically 0.25-1.25 μg/mL.
Source
E. coli-derived human GDF-7/BMP-12 protein
Thr322-Arg450
Accession #
N-terminal Sequence
Thr322
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
GDF7
Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
14 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in HCl.
Purity
>95%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human GDF-7 Protein, CF

  • BMP12
  • BMP-12
  • GDF7
  • GDF-7
  • growth differentiation factor 7
  • growth/differentiation factor 7

Background

Growth Differentiation Factor-7 (GDF-7; also called BMP-12 and CDMP-3) is a member of the BMP family of TGF-beta superfamily proteins (1, 2). GDF­7 is synthesized as a large precursor protein that consists of an N-­terminal 19 amino acid (aa) signal sequence, a 302 aa pro region and a 129 aa C-terminal mature peptide. At the amino acid level, mature human GDF-7 shares 85% and 88% aa sequence identity with mature GDF-7 in mouse and rat, respectively. Mature human GDF-7 lacks a glycine repeat that is found in both mouse and rat GDF-7. Based on sequence similarity, GDF-7 is categorized with GDF-5 and -6, as a subgroup within the BMP family. GDF-7 functions as a homodimer and elicits its bioactivity by mediating the formation of a heterodimeric receptor complex consisting of a type 1 (BMPR-IB) and a type II (BMPR-II or Activin RII) serine/threonine kinase receptor. GDF-7 signaling results in the phosphorylation and activation of cytosolic Smad proteins (Smad1, 5, and 8) (3, 4). GDF-7 is involved in tendon and ligament formation and repair (5-8). GDF-7 also regulates bone formation, mesenchymal stem cell differentiation, neuronal differentiation, and axon guidance in the central nervous system (9-12).
  1. Storm, E.E. et al. (1994) Nature 368:639.
  2. Hotten, G. et al. (1994) Biochem Biophys Res Commun 204:646.
  3. Nishitoh, H. et al. (1996) J Biol Chem 271:21345.
  4. Mueller, T.D. and J. Nickel (2012) FEBS Lett 586:1846.
  5. Fu, S.C. et al. (2003) Life Sci 72:2965.
  6. Gulati, B.R. et al. (2013) Cells Tissues Organs 198:377.
  7. Shen, H. et al. (2013) PLoS One 8:e77613.
  8. Sena, K. et al. (2003) J Dent Res 82:166.
  9. Butler, S.J. and J. Dodd (2003) Neuron 38:389.
  10. Watakabe, A. et al. (2001) J Neurochem 76:1455.
  11. Lou, J. et al. (2001) J Orthop Res 19:1199.
  12. Wang, Q.W. et al. (2005) J Biosci Bioeng 100:418.

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Bioinformatics

Gene Symbol GDF7
Entrez
Uniprot