Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is typically 0.25-1.25 μg/mL.
E. coli-derived human GDF-7/BMP-12 protein Thr322-Arg450
<0.10 EU per 1 μg of the protein by the LAL method.
14 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in HCl.
>95%, by SDS-PAGE with silver staining.
Reconstitute at 500 μg/mL in 4 mM HCl.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human GDF-7 Protein, CF
growth differentiation factor 7
growth/differentiation factor 7
Growth Differentiation Factor-7 (GDF-7; also called BMP-12 and CDMP-3) is a member of the BMP family of TGF-beta superfamily proteins (1, 2). GDF7 is synthesized as a large precursor protein that consists of an N-terminal 19 amino acid (aa) signal sequence, a 302 aa pro region and a 129 aa C-terminal mature peptide. At the amino acid level, mature human GDF-7 shares 85% and 88% aa sequence identity with mature GDF-7 in mouse and rat, respectively. Mature human GDF-7 lacks a glycine repeat that is found in both mouse and rat GDF-7. Based on sequence similarity, GDF-7 is categorized with GDF-5 and -6, as a subgroup within the BMP family. GDF-7 functions as a homodimer and elicits its bioactivity by mediating the formation of a heterodimeric receptor complex consisting of a type 1 (BMPR-IB) and a type II (BMPR-II or Activin RII) serine/threonine kinase receptor. GDF-7 signaling results in the phosphorylation and activation of cytosolic Smad proteins (Smad1, 5, and 8) (3, 4). GDF-7 is involved in tendon and ligament formation and repair (5-8). GDF-7 also regulates bone formation, mesenchymal stem cell differentiation, neuronal differentiation, and axon guidance in the central nervous system (9-12).
Storm, E.E. et al. (1994) Nature 368:639.
Hotten, G. et al. (1994) Biochem Biophys Res Commun 204:646.
Nishitoh, H. et al. (1996) J Biol Chem 271:21345.
Mueller, T.D. and J. Nickel (2012) FEBS Lett 586:1846.
Fu, S.C. et al. (2003) Life Sci 72:2965.
Gulati, B.R. et al. (2013) Cells Tissues Organs 198:377.
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