BMP-7 Antibody (164313) [Unconjugated]

354-BP) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑7 (1 µg/mL) is neutralized (green line) by increasing concentrations of Human BMP‑7 Monoclonal Antibody (Catalog # MAB3542). The ND₅₀ is typically 4-12 µg/mL in the presence of L-ascorbic acid (50 µg/mL)." > 354-BP) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑7 (1 µg/mL) is neutralized (green line) by increasing concentrations of Human BMP‑7 Monoclonal Antibody (Catalog # MAB3542). The ND₅₀ is typically 4-12 µg/mL in the presence of L-ascorbic acid (50 µg/mL)." title="Recombinant Human BMP‑7 (Catalog # 354-BP) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑7 (1 µg/mL) is neutralized (green line) by increasing concentrations of Human BMP‑7 Monoclonal Antibody (Catalog # MAB3542). The ND₅₀ is typically 4-12 µg/mL in the presence of L-ascorbic acid (50 µg/mL)." />

Recombinant Human BMP‑7 (Catalog # <a class=

CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="BMP‑7 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 25 µg/mL Human BMP‑7 Monoclonal Antibody (Catalog # MAB3542) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." />

BMP‑7 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 25 µg/mL Human BMP‑7 Monoclonal Antibody (Catalog # MAB3542) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." > CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." title="BMP-7 was detected in immersion fixed paraffin-embedded sections of human prostate using Human BMP-7 Monoclonal Antibody (Catalog # MAB3542) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." />

BMP-7 was detected in immersion fixed paraffin-embedded sections of human prostate using Human BMP-7 Monoclonal Antibody (Catalog # MAB3542) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

354-BP) was serially diluted and captured by Mouse Anti-Human BMP‑7 Monoclonal Antibody (Catalog # MAB3542) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human BMP‑7 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" > 354-BP) was serially diluted and captured by Mouse Anti-Human BMP‑7 Monoclonal Antibody (Catalog # MAB3542) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human BMP‑7 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" title="Recombinant Human BMP‑7 (Catalog # 354-BP) was serially diluted and captured by Mouse Anti-Human BMP‑7 Monoclonal Antibody (Catalog # MAB3542) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human BMP‑7 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" />

Recombinant Human BMP‑7 (Catalog # <a class=

• Reactivity: Hu
• Applications: IHC, B/N, ELISA(Cap)
• Clone: 164313
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

BMP-7 Antibody (164313) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human BMP‑7
  Arg292-His431
  Accession # P18075
• Specificity: Detects human BMP-7 in ELISAs. In ELISAs, this antibody shows approximately 25-50% cross-reactivity with recombinant human (rh) BMP‑6 and no cross-reactivity with rhBMP-2, -3, -4, -5, or -8.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BMP7
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 8-25 ug/mL
  • Neutralization 4-12 ug/mL
• Application Notes: ELISA Detection: Human BMP-7 Biotinylated Antibody (Catalog number BAM354)
  Standard: Recombinant Human BMP-7 (Catalog number 354-BP)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BMP-7 Antibody (164313) [Unconjugated]

• BMP7
• BMP-7
• bone morphogenetic protein 7
• Eptotermin alfa
• OP-1
• OP-1OP1
• Osteogenic protein 1

Background

BMP-7, or osteogenic protein 1 (OP-1), is one of at least 15 structurally and functionally related BMPs, which are members of the transforming growth factor beta (TGF-beta ) superfamily. BMPs regulate cartilage and bone formation, embryogenesis and morphogenesis of various tissues and organs, and growth, differentiation, chemotaxis and apoptosis of various cell types. Biologically active BMP-7 is a disulfide-linked homodimer. Cellular responses to BMP-7 have been shown to be mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BMP-7 Antibody (MAB3542)(8)

Publications using MAB3542

• RL Cate, N di Clement, C Racine, NP Groome, RB Pepinsky, A Whitty The anti-M�llerian hormone prodomain is displaced from the hormone/prodomain complex upon bivalent binding to the hormone receptor The Journal of Biological Chemistry, 2021-11-19;0(0):101429. 2021-11-19 [PMID: 34801555] (ELISA Detection, Human)
• Seonghun Kim, Cheol-Hee Jeong, Sang Hyun Song, Jo Eun Um, Hyun Sil Kim, Jun Seop Yun et al. Micellized Protein Transduction Domain-Bone Morphogenetic Protein-7 Efficiently Blocks Renal Fibrosis Via Inhibition of Transforming Growth Factor-Beta–Mediated Epithelial–Mesenchymal Transition Frontiers in Pharmacology 11/19/2020 [PMID: 33364962]
• Aurore Bouty, Kelly Walton, Nurin Aisyiyah Listyasari, Gorjana Robevska, Jocelyn Van den Bergen, Ardy Santosa et al. Functional Characterization of Two New Variants in the Bone Morphogenetic Protein 7 Prodomain in Two Pairs of Monozygotic Twins With Hypospadias Journal of the Endocrine Society 4/1/2019 [PMID: 30963139]
• Wohl A, Troilo H, Collins R, Baldock C, Sengle G Extracellular Regulation of Bone Morphogenetic Protein Activity by the Microfibril Component Fibrillin-1. J Biol Chem, 2016-04-08;291(24):12732-46. 4/8/2016 [PMID: 27059954]
• Valérie Camara-Clayette, Serge Koscielny, Sébastien Roux, Thierry Lamy, Jacques Bosq, Marc Bernard et al. BMP7 Expression Correlates with Secondary Drug Resistance in Mantle Cell Lymphoma PLoS ONE 9/12/2013 [PMID: 24069261]
• Imran Khan, Neeraj Kumar, Ila Pant, Sivakrishna Narra, Paturu Kondaiah Activation of TGF-beta Pathway by Areca Nut Constituents: A Possible Cause of Oral Submucous Fibrosis PLoS ONE 12/19/2012 [PMID: 23284772]
• Péter Csomor, Balázs Liktor, Bálint Liktor, Zoltán Szekanecz, István Sziklai, Tamás Karosi Expression of bone morphogenetic protein 2, 4, 5, and 7 correlates with histological activity of otosclerotic foci Acta Oto-Laryngologica 6/1/2012 [PMID: 22385409]
• Dai J, Keller J, Zhang J, Lu Y, Yao Z, Keller ET Bone morphogenetic protein-6 promotes osteoblastic prostate cancer bone metastases through a dual mechanism. Cancer Res., 2005-09-15;65(18):8274-85. 2005-09-15 [PMID: 16166304] (Western Blot, Human)

Bioinformatics

• Gene Symbol: BMP7
• Uniprot:
  • P18075()