Recombinant Human BMP‑4 (Catalog # 314-BP) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by ...read more
Mouse myeloma cell line NS0-derived recombinant human BMP‑4 Ser293-Arg408 Accession # P12644
Detects human BMP-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human (rh) BMP‑2 and 10% cross-reactivity with rhBMP-5 and rhBMP-6 is observed.
<0.30 EU per 1 μg of the antibody by the LAL method.
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for BMP-4 Antibody [Unconjugated]
Bone morphogenetic protein 2B
bone morphogenetic protein 4
BMP-4 is a TGF-beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1 - 5). The human BMP-4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfide-linked homodimeric BMP-4 and facilitates its secretion. Similar intracellular processes may lead to the formation and recreation of BMP4/BMP7 disulfide-linked heterodimer (7 - 9). Mature human and mouse BMP-4 share 98% aa sequence identity. Human BMP-4 shares 85% aa sequence identity with human BMP-2 and less than 50% with other human BMPs. Compared to BMP-4 homodimers, BMP-4/BMP-7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP-4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPR‑IA) and type II (primarily Activin RIIA or BMPR-II) receptors (11, 12). The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans (13 - 15).
Zhang, P. et al. (2008) Blood 111:1933.
Gambaro, K. et al. (2006) Cell Death Differ. 13:1075.
Simic, P. and S. Vukicevic (2005) Cytokine Growth Factor Rev. 16:299.
Sadlon, T.J. et al. (2004) Stem Cells 22:457.
Frank, D.B. et al. (2005) Circ. Res. 97:496.
Wozney, J. et al. (1988) Science 242:1528.
Cui, Y. et al. (1998) EMBO J. 17:4735.
Cui, Y. et al. (2001) Genes Dev. 15:2797.
Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.
Chen, D. et al. (2004) Growth Factors 22:233.
Lavery, K. et al. (2008) J. Biol. Chem. April 24 epub.
Rosen, V. (2006) Ann. N.Y. Acad. Sci. 1068:19.
Jones, C.M. and J.C. Smith (1998) Dev. Biol. 194:12.
Takada, T. et al. (2003) J. Biol. Chem. 278:43229.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our BMP-4 Antibody [Unconjugated] and receive a gift card or discount.