BMP-4 Antibody (66119.11)

Product Discontinued

BMP-4 Antibody (66119.11) Summary

• Immunogen: Recombinant full length protein expressed in NSO cells (Human).
• Localization: Secreted, extracellular matrix.
• Specificity: Monoclonal Anti-BMP-4 neutralizes human BMP-4, and recognizes recombinant human BMP-4 by ELISA and immunoblotting. The shows approximately 5% cross-reactivity with recombinant human BMP-2, recombinant mouse BMP RIA and BMP-RIB.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: BMP4
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • ELISA 0.5-1.0 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: I-ELISA: detects a limit of around 6 ng/well of human BMP-4. Neut: 50% of the bioactivity due to 0.5 µg/ml recombinant human BMP-4I-WB: Use human BMP-4 at 50 ng/lane under non-reducing and reducing conditions.
• Control:

  BMP-4 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 5% Trehalose
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for BMP-4 Antibody (66119.11)

• BMP2B
• BMP-2B
• BMP2B1
• BMP2BMCOPS6
• BMP4
• BMP-4
• Bone morphogenetic protein 2B
• bone morphogenetic protein 4
• DVR4
• MCOPS6
• OFC11
• ZYME

Background

Bone Morphogenic Proteins (BMP) are members of the TGF-beta superfamily that affect bone and cartilage formation (1,2,3). Mature BMPs are 30-38 kDa proteins that assume a TGF-beta -like cysteine knot configuration.Lovostatin increases bone formation by turning on the bmp-2 gene (4). BMPs stimulate the production of specific bone matrix proteins and alter stromal cell and osteoclast proliferation (5,6). BMPs may also be an important factor for development of the viscera, with roles in cell proliferation, apoptosis, differentiation, and morphogenesis (1,7).BMPs appear to be responsible for normal dorsal/ventral patterning. Like TGF-beta, BMPs bind to a type II receptor, which then recruits the transducing type I receptor unit, activating the Smad protein signaling pathway (8,9,10).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BMP4
• Entrez:
  • 652(Human)