BMP-4 Overexpression Lysate (Native)


Western Blot: BMP4 Overexpression Lysate (Adult Normal) [NBL1-08002] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BMP4.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

BMP-4 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC219979 and based on accession number NM_130850. The protein contains a C-terminal DDK tag.
Homo sapiens bone morphogenetic protein 4 (BMP4), transcript variant 2, mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
46.4 kDa

Lysate Details for BMP-4

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for BMP-4 Overexpression Lysate (Native)

  • BMP-2B
  • BMP2B1
  • BMP4
  • BMP-4
  • Bone morphogenetic protein 2B
  • bone morphogenetic protein 4
  • DVR4
  • OFC11
  • ZYME


The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein. [provided by RefSeq]. Transcript Variant: This variant (2) utilizes exons 1, 3, 4 and 5 but uses an alternate splice acceptor site on exon 1, resulting in a shorter transcript compared to variant 1. Variants 1, 2 and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for BMP-4 Lysate (NBL1-08002) (0)

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Video Protocols

WB Video Protocol

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Contact Information

Product PDFs


Gene Symbol BMP4

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