BMP-4 Antibody (1128D) [Unconjugated] (MAB5020)

Images

 
Western blot shows Recombinant Mouse BMP‑4 (Catalog # 5020-BP) and lysates of mouse embryo tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Mouse BMP‑4 Monoclonal Antibody (Catalog # MAB5020) ...read more
BMP‑4 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Rabbit Anti-Mouse BMP‑4 Monoclonal Antibody (Catalog # MAB5020) at 1:125 dilution overnight at 4 °C. Tissue was stained using ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clone
1128D
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

BMP-4 Antibody (1128D) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Chinese hamster ovary cell line CHO-derived mature mouse BMP-4
Ser293-Arg408
Accession # P21275
Specificity
Detects mouse BMP-4 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
BMP4
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000 dilution
  • Immunohistochemistry 1:125 dilution

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
Sodium Azide

Notes

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BMP-4 Antibody (1128D) [Unconjugated]

  • BMP-2B
  • BMP2B1
  • BMP2BMCOPS6
  • BMP4
  • BMP-4
  • Bone morphogenetic protein 2B
  • bone morphogenetic protein 4
  • DVR4
  • OFC11
  • ZYME

Background

BMP-4 is a TGF-beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1-5). The mouse BMP-4 precursor contains a 273 amino acid (aa) propeptide and a 116 aa mature protein (6). The propeptide is cleaved intracellularly by furin or proprotein convertase 6, enabling the 15 kDa mature BMP-4 monomer to form an active disulfide linked homodimer or heterodimer with BMP-7 (7-9). Mature mouse and human BMP-4 share 98% aa sequence identity. Mouse BMP-4 shares 85% aa sequence identity with mouse BMP-2 and 35%-54% with other mouse BMPs. Compared to BMP-4 homodimers, BMP-4/BMP-7 heterodimers exhibit a greater potency in inducing osteogenic differentiation (9). In Xenopus, the heterodimers can also induce the formation of mesoderm, whereas BMP-4 homodimers only provide ventralizing signals for existing mesoderm (10). BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPR-IA) and type II (primarily Activin RIIA or BMPR-II) receptors (11, 12). The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans (13-15).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BMP4
Entrez
Uniprot