Recombinant Human BMP-2 Protein

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SDS-Page: Human BMP-2 Protein [NBP2-34928] - The Recombinant Human BMP-2 homodimeric protein is resolved by SDS-PAGE under reducing (R) and non-reducing (NR) conditions.
Bioactivity: Recombinant Human BMP-2 Protein [NBP2-34928]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Format
Carrier-Free
Concentration
LYOPH

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Catalog# & Formulation Size Price

Recombinant Human BMP-2 Protein Summary

Description
A biologically active homodimeric protein consisting of two 115 residues non-glycosylated polypeptide chains and corresponding to BMP2.

Source: E. coli

Amino Acid Sequence: MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPFPLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVEGCGCR

Preparation
Method
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
Details of Functionality
BMP2 protein is fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 200 ng/ml, corresponding to a specific activity of > 5.0 x 10^3 IU/mg.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
BMP2
Purity
>95% pure by SDS-PAGE
Endotoxin Note
Less than 1 EU/ug of endotoxin as determined by LAL method.

Applications/Dilutions

Theoretical MW
26.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NBP2-34928.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered concentrated solution containing 10 mM sodium citrate pH 3.5.
Concentration
LYOPH
Purity
>95% pure by SDS-PAGE
Reconstitution Instructions
Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 - 1.0 mg/ml.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Human BMP-2 Protein

  • BMP2
  • BMP-2
  • BMP-2A
  • BMP2ABone morphogenetic protein 2A
  • bone morphogenetic protein 2

Background

Bone morphogenetic protein 2 plays a dominant role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration. Mature BMP-2 forms disulfide linked homodimers and heterodimers with BMP-7. BMP-2 induces cartilage and bone formation, and BMP-2/BMP-7 heterodimers are even more potent than BMP-2 homodimers. BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine production, and is required for cardiac contractility.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Publications for BMP-2 Biologically Active Protein (NBP2-34928)(2)

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FAQs for BMP-2 Biologically Active Protein (NBP2-34928). (Showing 1 - 2 of 2 FAQs).

  1. I am interested in Bone Morphogenic Protein 2 (BMP-2) for clinical trial in humans. I am using this protein in stem cells culture for future use in humans. Do you have BMP-2 in pharma grade that I can use for humans?
    • Our antibodies are for research purposes only unfortunately. I am sorry we cannot be of further help.
  2. Can you protein fluoresence labeled BMP2 protein?
    • Unfortunately, we don't custom make any fluorophore conjugation to protein, but only making fluorophore-antibody conjugation in house. This is because proteins diverse a lot, with the different number of sites that can be conjugated with fluorophores and thus the efficiency and the successful rate cannot be determined.

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Bioinformatics

Gene Symbol BMP2
Entrez
Uniprot