Vang-like Protein 2/VANGL2 Antibody

Western blot shows lysates of mouse kidney, human and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Vang-like Protein 2/VANGL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4815) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Vang-like Protein 2/VANGL2 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Vang-like Protein 2/VANGL2 was detected in immersion fixed frozen sections of mouse embryo (E15.5) using Sheep Anti-Human/Mouse/Rat Vang-like Protein 2/VANGL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4815) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to developing spinal cord. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Vang-like Protein 2/VANGL2 Antibody Summary

• Immunogen: E. coli-derived recombinant human Vang-like Protein 2/VANGL2
  Gln241-Val521
  Accession # Q9ULK5
• Specificity: Detects human, mouse, and rat Vang-like Protein 2/VANGL2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human VANGL1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: VANGL2
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Vang-like Protein 2/VANGL2 Antibody

• KIAA1215Loop-tail protein 1 homolog
• loop-tail-associated protein
• LPP1
• LTAP
• LTAPVan Gogh-like protein 2
• MGC119403
• MGC119404
• STB1
• STB1Strabismus 1
• STBM
• STBM1
• Strabismus
• vang (van gogh, Drosophila)-like 2, vang, van gogh-like 2 (Drosophila)
• Vang like Protein 2
• VANGL2
• vang-like 2 (van gogh, Drosophila)
• Vang-like Protein 2

Background

VANGL2 (van Gogh-like protein 2; also Strabismus and Ltap) is a 65 kDa member of the Vang family of proteins. It is found in the membranes of embryonic neurons and epithelium where it associates with DVL1-3 and various frizzleds. VANGL2 mutations are associated with neural tube defects. Human VANGL2 is a 521 amino acid (aa) 4-transmembrane protein that contains an N-terminal (aa 1‑108) and C-terminal (aa 239‑521) cytoplasmic domain. There is a Ser-rich region in the N-terminus (aa 5‑20) and a PDZ-binding motif over aa 506‑521. Over aa 241‑521, human, mouse and rat show total identity in amino acid sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Vang-like Protein 2/VANGL2 Antibody (AF4815)(7)

Publications using AF4815

• Y Ban, T Yu, B Feng, C Lorenz, X Wang, C Baker, Y Zou Prickle promotes the formation and maintenance of glutamatergic synapses by stabilizing the intercellular planar cell polarity complex Science Advances, 2021-10-06;7(41):eabh2974. 2021-10-06 [PMID: 34613779] (Western Blot, Mouse)
• X Cheng, L Song, M Lan, B Shi, J Li Morphological and molecular comparisons between tibialis anterior muscle and levator veli palatini muscle: A preliminary study on their augmentation potential Exp Ther Med, 2017-10-30;15(1):247-253. 2017-10-30 [PMID: 29375687] (Western Blot, Rat)
• W Luo, H Yi, J Taylor, JD Li, F Chi, NW Todd, X Lin, D Ren, P Chen Cilia distribution and polarity in the epithelial lining of the mouse middle ear cavity Sci Rep, 2017-03-30;7(0):45870. 2017-03-30 [PMID: 28358397] (IHC, Mouse)
• C Dyberg, P Papachrist, BH Haug, H Lagercrant, P Kogner, T Ringstedt, M Wickström, JI Johnsen Planar cell polarity gene expression correlates with tumor cell viability and prognostic outcome in neuroblastoma BMC Cancer, 2016-03-31;16(1):259. 2016-03-31 [PMID: 27036398] (Western Blot, Human)
• VANGL2 regulates membrane trafficking of MMP14 to control cell polarity and migration. J. Cell. Sci., 2012-02-22;125(0):2141-7. 2012-02-22 [PMID: 22357946] (ICC, Human)
• Trichas G, Joyce B, Crompton LA, Wilkins V, Clements M, Tada M, Rodriguez TA, Srinivas S Nodal dependent differential localisation of dishevelled-2 demarcates regions of differing cell behaviour in the visceral endoderm. PLoS Biol., 2011-02-22;9(2):e1001019. 2011-02-22 [PMID: 21364967] (IHC-Fr, Mouse)
• Lindqvist M, Horn Z, Bryja V, Schulte G, Papachristou P, Ajima R, Dyberg C, Arenas E, Yamaguchi TP, Lagercrantz H, Ringstedt T Vang-like protein 2 and Rac1 interact to regulate adherens junctions. J. Cell. Sci., 2010-01-12;123(0):472-83. 2010-01-12 [PMID: 20067994] (ICC, Immunoprecipitation, Human)

Bioinformatics

• Gene Symbol: VANGL2
• Uniprot:
  • Q9ULK5()