Recombinant Human BLIMP1/PRDM1 GST (N-Term) Protein

SDS-Page: Recombinant Human BLIMP1/PRDM1 Protein [H00000639-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

• Reactivity: Hu
• Applications: WB, ELISA, MA, AP

Recombinant Human BLIMP1/PRDM1 GST (N-Term) Protein Summary

• Description: A recombinant protein with a N-terminal GST tag corresponding to the amino acid sequence 1-109 of Human BLIMP1/PRDM1
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: MKMDMEDADMTLWTEAEFEEKCTYIVNDHPWDSGADGGTSVQAEASLPRNLLFKYATNSEEVIGVMSKEYIPKGTRFGPLIGEIYTNDTVPKNANRKYFWRIYSRGELH

• Preparation Method: in vitro wheat germ expression system
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Recombinant Protein
• Gene: PRDM1
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • Western Blot
• Theoretical MW: 37.73 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human BLIMP1/PRDM1 GST (N-Term) Protein

• Beta-interferon gene positive regulatory domain I-binding factor
• beta-interferon gene positive-regulatory domain I binding factor
• BLIMP1
• BLIMP-1
• BLIMP1MGC118925
• blmp-1
• B-lymphocyte-induced maturation protein 1
• MGC118922
• MGC118923
• Positive regulatory domain I-binding factor 1
• PR domain containing 1, with ZNF domain
• PR domain zinc finger protein 1
• PR domain-containing protein 1
• PRDI-BF1MGC118924
• PRDI-binding factor 1
• PRDI-binding factor-1
• PRDM1
• PR-domain zinc finger protein 1
• ZNFPR1A1

Background

PRDM1 - PR domain containing 1, with ZNF domain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for BLIMP1/PRDM1 Partial Recombinant Protein (H00000639-Q01). (Showing 1 - 1 of 1 FAQ).

1. Will this protein bind DNA? I would like to use it in gel-shift assays. It says it comprises zinc finger domain, amino acids 1-110, but unclear to me if it can bind DNA.
   • This product is produced by a Taiwanese company called Abnova and we distribute it for them. We do no in-house testing or development of their products. They do not test the activity of their recombinant proteins. The proteins are produced in a cell-free wheat germ system with an N-terminal 26kDa GST-tag. Due to this expression system, in our experience, the proteins do not always undergo the same folding and post-transnational modifications they might undergo in an endogenous cell. Because of this, we do not recommend this protein for use in functional assays and do not guarantee them for this use. They are mainly intended for use in Western Blots as a positive control with their antibody. While some of their proteins do show biological activity, unfortunately, because they are not directly tested for this, there is no way to predict whether or not this particular product shows biological activity.

Bioinformatics

• Gene Symbol: PRDM1