BIN1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: BIN1 Antibody [NBP2-15587] - BIN1 antibody detects BIN1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BIN1 stained by BIN1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.

Immunohistochemistry-Paraffin: BIN1 Antibody [NBP2-15587] - BIN1 antibody detects BIN1 protein at cytoplasm in human papillary adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human papillary adenocarcinoma. BIN1 antibody diluted at 1:500.

Western Blot: BIN1 Antibody [NBP2-15587] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BIN1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: BIN1 Antibody [NBP2-15587] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BIN1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: BIN1 Antibody [NBP2-15587] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

BIN1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit BIN1 Antibody - BSA Free (NBP2-15587) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human BIN1. The exact sequence is proprietary.
• Localization: Isoform BIN1: Nucleus; Isoform IIA: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BIN1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (80%), Chicken (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for BIN1 Antibody - BSA Free

• AMPH2
• Amphiphysin II
• Amphiphysin-like protein
• AMPHLDKFZp547F068
• box dependant MYC interacting protein 1
• bridging integrator 1Box-dependent myc-interacting protein 1
• MGC10367
• myc box-dependent-interacting protein 1
• SH3P9

Background

This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynanim, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: BIN1