BIM Long Antibody

BIMLwas detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human BIMLLong (BIM L) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1325) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and perinuclear area in monocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

BIM Long Antibody Summary

• Immunogen: E. coli-derived recombinant human BIM_L Long (BIM L)
  Ala2-Arg120
  Accession # NP_006529
• Specificity: Detects human BIM_L in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BCL2L11
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BIM Long Antibody

• BAM
• BCL2L11
• BIM Long
• BIM
• BOD

Background

BIM_L (also known as Bod) is one of several splice variants of BIM, a pro-apoptotic protein belonging to the BH3 domain-only subgroup of Bcl-2 family members. BIM_L is thought to promote apoptosis by binding and inhibiting the activity of anti-apoptoticBcl-2 family members, thereby inducing the release of cytochrome c from mitochondria. BIM_L is normally sequestered in an inactive conformation from anti-apoptotic Bcl-2 family members through binding to the microtubule-associated dynein motor complex.Certain apoptotic stimuli release BIM_L from microtubules to neutralize anti-apoptotic Bcl-2 family members, allowing for the initiation of apoptosis.

1. O'Connor, L. et al. (1998) EMBO J. 17:384.
2. Puthalakath, H. et al. (1999) Mol. Cell. 3:287.
3. Miyashita, T. (2001) FEBS Letters 509:135.
4. Strasser, A. (2000) Ann N Y Acad Sci. 917:541.
5. Marani, M. et al. (2002) Mol. Cell. Biol. 22:3577.

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCL2L11
• Uniprot:
  • NP_006529()