Biglycan Antibody (303203)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
303203
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Biglycan Antibody (303203) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Biglycan
Asp38-Lys368
Accession # P21810
Specificity
Detects human Biglycan in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BGN
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publication using
MAB2667 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Biglycan Antibody (303203)

  • BGN
  • Biglycan
  • Bone/cartilage proteoglycan I
  • bone/cartilage proteoglycan-I
  • dermatan sulphate proteoglycan I
  • DSPG1
  • PGI
  • PG-S1
  • SLRR1A
  • SLRR1Abiglycan proteoglycan
  • small leucine-rich protein 1A

Background

Biglycan, also known as PG I, is a secreted chondroitin/dermatan sulfate proteoglycan in the small leucine-rich proteoglycan (SLRP) family. SLRP family members are characterized by N-terminal and C-terminal cysteine-rich regions that flank a central region containing 10 - 12 tandem leucine-rich repeats (LRRs). Biglycan function is important in the development and maintenance of many tissues (1, 2). The human Biglycan cDNA encodes a 368 amino acid (aa) precursor with a 19 aa signal sequence and a 18 aa propeptide that is cleaved by BMP-1 (3). Mature Biglycan contains N-linked glycosylation in addition to two glycosaminoglycan (GAG) chains (4). The 45 kDa core protein is approximately one third the molecular weight of the fully glycanated form and can assemble into noncovalently-associated dimers (5). Human Biglycan shares 97% aa sequence identity with bovine, mouse, and rat Biglycan and 57% aa sequence identity with human Decorin. Biglycan binds several matrix proteins, including fibrillar collagens, Matrilin-1, and beta IG-H3 (6 - 8). Its multiple LRRs, N-linked glycosylation, and GAG chains mediate distinct interactions that enable extensive crosslinking and stabilization of the collagen matrix (6 - 8). Mature Biglycan can be further cleaved by proteases, resulting in non-glycanated fragments (9). In osteoarthritis, MMP-13-induced breakdown of Biglycan likely interferes with collagen crosslinking and contributes to cartilage degradation (10). Biglycan also binds and modulates the activity of a variety of non-matrix proteins, including BMP-4, C1q, collectins, TGF-beta, TNF-alpha, and WISP-1 (11, 12). Biglycan functions as a proinflammatory mediator by binding TLR2 and TLR4 on macrophages and inducing TNF-alpha and MIP-2 production (13). Biglycan knockout mice have compromised inflammatory responses and are resistant to LPS-induced septic shock (13). Biglycan binds HDL/Apolipoprotein E complexes in atherosclerotic plaques and also binds SR-A, blocking the uptake and degradation of LDL (14, 15).

  1. Hocking, A.M. et al. (1998) Matrix Biol. 17:1.
  2. Yoon, J.H. and J. Halper (2005) J. Musculoskelet. Neuronal Interact. 5:22.
  3. Scott, I.C. et al. (2000) J. Biol. Chem. 275:30504.
  4. Fisher, L.W. et al. (1989) J. Biol. Chem. 264:4571.
  5. Scott, P.G. et al. (2006) J. Biol. Chem. 281:13324.
  6. Schonherr, E. et al. (1995) J. Biol. Chem. 270:2776.
  7. Wiberg, C. et al. (2003) J. Biol. Chem. 278:37698.
  8. Reinboth, B. et al. (2006) J. Biol. Chem. 281:7816.
  9. Roughley, P.J. et al. (1996) Biochem. J. 318:779.
  10. Monfort, J. et al. (2006) Arthritis Res. Ther. 8:R26.
  11. Groeneveld, T.W.L. et al. (2005) J. Immunol. 175:4715.
  12. Moreno, M. et al. (2005) EMBO J. 24:1397.
  13. Schaefer, L. et al. (2005) J. Clin. Invest. 115:2223.
  14. Olin, K.L. et al. (2001) Arterioscler. Thromb. Vasc. Biol. 21:129.
  15. Santiago-Garcia, J. et al. (2003) J. Biol. Chem. 278:6942.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Biglycan Antibody (MAB2667)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol BGN
Uniprot