Prostaglandin I2 Synthase Antibody (852307) [Unconjugated]

Images

 
Western blot shows lysates of SK‑OV‑3 human ovarian adenocarcinoma cell line and human ovary tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Prostaglandin I2 Synthase Monoclonal Antibody (Catalog ...read more
Simple Western lane view shows lysates of SK‑OV‑3 human ovarian adenocarcinoma cell line and human ovarian cancer, loaded at 0.5 mg/mL. A specific band was detected for Prostaglandin I2 Synthase at approximately 55 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clone
852307
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Prostaglandin I2 Synthase Antibody (852307) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Prostaglandin I2 Synthase
Arg21-Pro500
Accession # Q16647
Specificity
Detects human Prostaglandin I2 Synthase in ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PTGIS
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using
MAB7788 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Prostaglandin I2 Synthase Antibody (852307) [Unconjugated]

  • CYP8
  • CYP8A1
  • CYP8A1PTGI
  • CYP8prostacyclin synthase
  • EC 5.3.99.4
  • MGC126858
  • MGC126860
  • PGIS
  • PGIScytochrome P450, family 8, subfamily A, polypeptide 1
  • Prostacyclin Synthase
  • prostaglandin I2 (prostacyclin) synthase
  • Prostaglandin I2 synthase
  • PTGI
  • PTGIS

Background

PTGIS (Prostacyclin synthase; also CYP8A1, PGIS and Prostaglandin I2 synthase) is a 50-52 kDa (57 kDa predicted) microsomal, integral membrane member of Family 8 in the cytochrome P450 superfamily of molecules. It is expressed by skeletal and smooth muscle, fibroblasts, macrophages and endothelium. Prostaglandin I2/prostacyclin and thromboxane A2/TXA2 are two lipid mediators that are derived from the same precursor (PGH2). Acting upon PGH2, TXA2 is created through the action of TX synthase, while PGI2 is generated through the action of PTGIS/PGIS. Although they share a common precursor, they exhibit opposite effects on the vasculature, with PGI2 reported to block platelet aggregation, induce vasodilation, and inhibit smooth muscle proliferation. It apparently does so by binding to either the IP receptor or PPAR gamma . Human PTGIS is 500 amino acids (aa) in length. It is a single span transmembrane protein that contains an N-terminal transmembrane segment (aa 1-20) coupled to a lengthy 480 aa cytoplasmic region. Over aa 21-500, human PTGIS shares 86% aa sequence identity with mouse PTGIS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Prostaglandin I2 Synthase Antibody (MAB7788)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Video Protocols

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Bioinformatics

Gene Symbol PTGIS
Uniprot