Biglycan Antibody (303203) [Alexa Fluor® 405]

• Applications: WB
• Clone: 303203
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

Biglycan Antibody (303203) [Alexa Fluor® 405] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Biglycan
  Asp38-Lys368
  Accession # P21810
• Specificity: Detects human Biglycan in direct ELISAs and Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Biglycan, also known as PG I, is a secreted chondroitin/dermatan sulfate proteoglycan in the small leucine-rich proteoglycan (SLRP) family. SLRP family members are characterized by N-terminal and C-terminal cysteine-rich regions that flank a central region containing 10 - 12 tandem leucine-rich repeats (LRRs). Biglycan function is important in the development and maintenance of many tissues (1, 2). The human Biglycan cDNA encodes a 368 amino acid (aa) precursor with a 19 aa signal sequence and a 18 aa propeptide that is cleaved by BMP-1 (3). Mature Biglycan contains N-linked glycosylation in addition to two glycosaminoglycan (GAG) chains (4). The 45 kDa core protein is approximately one third the molecular weight of the fully glycanated form and can assemble into noncovalently-associated dimers (5). Human Biglycan shares 97% aa sequence identity with bovine, mouse, and rat Biglycan and 57% aa sequence identity with human Decorin. Biglycan binds several matrix proteins, including fibrillar collagens, Matrilin-1, and beta IG-H3 (6 - 8). Its multiple LRRs, N-linked glycosylation, and GAG chains mediate distinct interactions that enable extensive crosslinking and stabilization of the collagen matrix (6 - 8). Mature Biglycan can be further cleaved by proteases, resulting in non-glycanated fragments (9). In osteoarthritis, MMP-13-induced breakdown of Biglycan likely interferes with collagen crosslinking and contributes to cartilage degradation (10). Biglycan also binds and modulates the activity of a variety of non-matrix proteins, including BMP-4, C1q, collectins, TGF-beta , TNF-alpha , and WISP-1 (11, 12). Biglycan functions as a proinflammatory mediator by binding TLR2 and TLR4 on macrophages and inducing TNF-alpha and MIP-2 production (13). Biglycan knockout mice have compromised inflammatory responses and are resistant to LPS-induced septic shock (13). Biglycan binds HDL/Apolipoprotein E complexes in atherosclerotic plaques and also binds SR-A, blocking the uptake and degradation of LDL (14, 15).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.