Betacellulin/BTC Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, IHC, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Betacellulin/BTC Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant mouse Betacellulin/BTC
  Asp32-Gln118
  Accession # Q543J8
• Specificity: Detects mouse Betacellulin/BTC in ELISAs and Western blots. In sandwich immunoassays, less than 0.02% cross-reactivity with recombinant mouse (rm) ErbB4, rmEGF R, rmAR, recombinant human (rh) BTC, rhTGF-alpha , and rhHB-EGF is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BTC
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse Betacellulin/BTC Antibody (Catalog # MAB10251) ELISA Detection: Mouse Betacellulin/BTC Biotinylated Antibody (Catalog # BAF1025) Standard: Recombinant Mouse Betacellulin/BTC (Catalog # 1025-CE)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Betacellulin/BTC Antibody [Biotin]

• Betacellulin
• BTC
• probetacellulin

Background

Betacellulin (BTC) is a member of the EGF family of cytokines which includes EGF, TGF-alpha , amphiregulin, HB-EGF, epiregulin, tomoregulin and the neuregulins. All EGF famiy members are synthesized as type I transmembrane precursor proteins containing one or more EGF-like domains in their extracellular region (1). BTC, a heparin-binding protein, was originally isolated from the conditioned media of mouse pancreatic beta tumor cells as a 32 kDa glycoprotien (2). The mouse BTC cDNA encodes a 177 amino acid (aa) residue precursor with a 31 aa signal peptide, an 87 aa residue extracellular region containing one EGF-like domain, a 21 aa transmembrane domain and a 38 aa cytoplasmic domain. Soluble BTC is released from the transmembrane precursor by proteolytic processing (3). Mouse BTC shares 93% and 79% aa sequence identity with rat and human BTC, respectively (1). The mouse BTC gene is tightly linked to that of amphiregulin on mouse chromosome 5 (4). BTC is expressed in most tissues including kidney, uterus, liver and pancreas. It is also present in body fluids including serum, milk and colostrum (5). BTC promotes pancreatic beta-cell growth and differentiation (6) and is a potent mitogen for retinal pigment epithelial cells, vascular smooth muscle cells and fibroblasts (1). The effects of BTC is mediated by binding to ErbB1 and ErbB4 homodimers as well as ErbB heterodimers (1).

1. Dunbar, A.J. and C. Goddard (2000) Int. J. Biochem. Cell Biol. 32:805.
2. Shing, Y. et al. (1993) Science 259:1604.
3. Tada, H. et al. (1999) J. Cell Biochem. 72:423.
4. Pathak, B.G. et al. (1995) Genomics 28:116.
5. Bastian, S.E. et al. (2001) J. Endocrinol. 168:203.
6. Li, L. et al. (2001) Endocrinology 142:5379.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BTC
• Uniprot:
  • Q543J8()