Recombinant Mouse beta-NGF Protein

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SDS-Page: Recombinant Mouse beta-NGF Protein [NBP2-76313] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel.
Bioactivity: Recombinant Mouse beta-NGF Protein [NBP2-76313] - beta-NGF induced TF-1 cell proliferation.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications PAGE, Bioactivity
Concentration
LYOPH

Order Details

Recombinant Mouse beta-NGF Protein Summary

Description
An un-tagged bioactive recombinant protein corresponding to Mouse beta-NGF

Source: E. coli

Amino Acid Sequence: MSSTHPVFHM GEFSVCDSVS VWVGDKTTAT DIKGKEVTVL AEVNINNSVF RQYFFETKCR ASNPVESGCR GIDSKHWNSY CTTTHTFVKA LTTDEKQAAW RFIRIDTACV CVLSRKATRR G

Specificity
beta-NGF is a dimer, 13.6/27.2 kDa (121/242 aa)
Preparation
Method
Protein is derived from genetically modified E. coli.
Details of Functionality
TF-1 cell proliferation, >2.0 x 10^5 units/mg (ED50 acceptance criteria are <5 ng/mL).
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
NGF
Purity
>95%, by SDS-PAGE
Endotoxin Note
Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
27.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Sterile water at 0.1 mg/mL

Notes

Lot Specific Information:
Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.

Detailed Reconstitution:
Centrifuge vial before opening. Suspend the product by gently pipetting the recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80C and avoid repeat freeze thaws.

Expiration Date:
12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.

Alternate Names for Recombinant Mouse beta-NGF Protein

  • Beta nerve growth factor precursor
  • beta-nerve growth factor
  • betaNGF
  • beta-NGF
  • HSAN5
  • MGC161426
  • nerve growth factor (beta polypeptide)
  • nerve growth factor, beta subunit
  • NGFB
  • NGFBMGC161428

Background

Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and some sensory neurons, and is composed by 3 types of subunits (alpha, beta and gamma) that specifically interact to form a 7S, 130 kDa molecular weight complex. The beta-NGF subunit is a secreted protein that forms a homodimer before being incorporated into the NGF complex. The human and mouse sequences are very similar and mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5). Deregulation of its expression has been associated with allergic rhinitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol NGF