Human beta-NGF Recombinant Protein

Product Discontinued

Human beta-NGF Recombinant Protein Summary

• Description: A single glycosylated polypeptide corresponding to amino acids Ser122 - Ala241 of NGF.
  
  

  Source: Mouse myeloma cell line, NS0

• Preparation Method: Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
• Details of Functionality: The ED50, as determined by a cell proliferation assay using human TF-1 cells, is less than 2 ng/ml.
• Source: Nso cells
• Protein/Peptide Type: Recombinant Protein
• Gene: NGF
• Purity: SDS-PAGE
• Endotoxin Note: Less than 1 EU/mg of the rHub-NGF as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 13.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: LYOPH
• Purity: SDS-PAGE
• Reconstitution Instructions: Reconstitute with PBS to a final concentration of 0.1 mg/ml.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Human beta-NGF Recombinant Protein

• Beta nerve growth factor precursor
• beta-nerve growth factor
• betaNGF
• beta-NGF
• HSAN5
• MGC161426
• nerve growth factor (beta polypeptide)
• nerve growth factor, beta subunit
• NGFB
• NGFBMGC161428

Background

Beta-NGF plays a critical role in the development of sympathetic and some sensory and cholinergic neurons. It has also been shown to promote differentiation, activation, and/or survival of mast cells, basophils, B cells, and neutrophils. It is expressed as a non-covalent homodimer that shows cross-species activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Publications for beta-NGF Recombinant Protein (NBP2-36437)(1)

Publication using NBP2-36437

• Dravid A, Raos B, Svirskis D, OCarroll SJ Optimised techniques for high-throughput screening of differentiated SH-SY5Y cells and application for neurite outgrowth assays Scientific reports 2021-12-14 [PMID: 34907283]

Bioinformatics

• Gene Symbol: NGF