Recombinant Human beta-NGF Protein

Product Discontinued

Recombinant Human beta-NGF Protein Summary

• Description: Recombinant bioactive protein for Human beta-NGF
  
  

  Source:CHO cells
  Amino Acid Sequence:(P01138)

• Preparation Method: Determined to be >98% pure by SDS-PAGE
• Details of Functionality: The ED50 as determined using PC12 cell line is 1.2-2 ng/ml and using DRG cell line is 0.8-1.5 ng/ml.
• Source: CHO
• Protein/Peptide Type: Recombinant Protein
• Gene: NGF
• Purity: >98%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 16.95 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Sterile filtered and then lyophilized in 30 mM Sodium Acetate, pH 5.5 containing 1% HAS.
• Concentration: Lyoph
• Purity: >98%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute in dH2O to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for long-term storage.

Alternate Names for Recombinant Human beta-NGF Protein

• Beta nerve growth factor precursor
• beta-nerve growth factor
• betaNGF
• beta-NGF
• HSAN5
• MGC161426
• nerve growth factor (beta polypeptide)
• nerve growth factor, beta subunit
• NGFB
• NGFBMGC161428

Background

Nerve growth factor-Beta (NGF-Beta ) is a potent neurotrophic factor that supports the growth and survivability of nerve and/or glial cells. The active form of human NGF-Beta is a dimer, formed by two identical 119 amino acid subunits, which is held together by strong hydrophobic interactions. Recombinant human BGF-b produced in CHO cells is a homodimer, glycosylated, polypeptide chain containing 2 identical 119 amino acids and having a molecular weight of 16.95 kDa. The protein is purified by proprietary chromatographic techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NGF