Human beta-NGF ELISA Kit (Colorimetric)

ELISA: Human beta-NGF ELISA Kit (Colorimetric) [NBP2-62776] - Samples were spiked with high concentrations of Human beta-NGF and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human beta-NGF ELISA Kit (Colorimetric) [NBP2-62776] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 15.63-1000 pg/mL
• Sensitivity: 9.38 pg/mL

Human beta-NGF ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human NGF in samples. No significant cross-reactivity or interference between Human NGF and analogues was observed.
• Standard Curve Range: 15.63-1000 pg/mL
• Sensitivity: 9.38 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.22%
• Intra-Assay: CV% < 4.55%
• Spike Recovery: 87-104%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: NGF

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human beta-NGF ELISA Kit (Colorimetric)

• Beta nerve growth factor precursor
• beta-nerve growth factor
• betaNGF
• beta-NGF
• HSAN5
• MGC161426
• nerve growth factor (beta polypeptide)
• nerve growth factor, beta subunit
• NGFB
• NGFBMGC161428

Background

Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and some sensory neurons, and is composed by 3 types of subunits (alpha, beta and gamma) that specifically interact to form a 7S, 130 kDa molecular weight complex. The beta-NGF subunit is a secreted protein that forms a homodimer before being incorporated into the NGF complex. The human and mouse sequences are very similar and mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5). Deregulation of its expression has been associated with allergic rhinitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for beta-NGF ELISA Kit (NBP2-62776)(1)

Publication using NBP2-62776

• Castelli V, d'Angelo M, Zazzeroni F et al. Intranasal delivery of NGF rescues hearing impairment in aged SAMP8 mice Cell death & disease 2023-09-13 [PMID: 37704645] (ELISA)

Bioinformatics

• Gene Symbol: NGF