Rat beta-NGF ELISA Kit (Colorimetric)

• Reactivity: Rt
• Applications: ELISA, NULL
• Suitable Sample Type: Cell Culture Supernatant, Serum
• Standard Curve Range: 15.6 to 1000 pg/mL
• Sensitivity: <1 pg/mL

Rat beta-NGF ELISA Kit (Colorimetric) Summary

• Description: Ngf (Rat) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of rat Ngf.
• Standard Curve Range: 15.6 to 1000 pg/mL
• Sensitivity: <1 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 6.1%
• Intra-Assay: CV% < 5.4%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: NGF

Applications/Dilutions

• Dilutions:
  • ELISA
  • Quantification

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Rat beta-NGF ELISA Kit (Colorimetric)

• Beta nerve growth factor precursor
• beta-nerve growth factor
• betaNGF
• beta-NGF
• HSAN5
• MGC161426
• nerve growth factor (beta polypeptide)
• nerve growth factor, beta subunit
• NGFB
• NGFBMGC161428

Background

Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and some sensory neurons, and is composed by 3 types of subunits (alpha, beta and gamma) that specifically interact to form a 7S, 130 kDa molecular weight complex. The beta-NGF subunit is a secreted protein that forms a homodimer before being incorporated into the NGF complex. The human and mouse sequences are very similar and mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5). Deregulation of its expression has been associated with allergic rhinitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for beta-NGF ELISA Kit (KA0401)(5)

Publications using KA0401

• Shimizu S, Saito M, Oiwa H et al. Olmesartan ameliorates urinary dysfunction in the spontaneously hypertensive rat via recovering bladder blood flow and decreasing oxidative stress. Neurourol Urodyn. 2014-03-01 [PMID: 23775684] (Rat)
• Inoue S, Saito M, Takenaka A. Hydroxyfasudil Ameliorates Bladder Dysfunction in Male Spontaneously Hypertensive Rats. Urology. 2012-02-24 [PMID: 22365452]
• Inoue S, Saito M, Tsounapi P et al. Effect of silodosin on detrusor overactivity in the male spontaneously hypertensive rat. BJU Int. 2011-12-07 [PMID: 22146017]
• Sanico AM, Stanisz AM, Gleeson TD et al. Nerve growth factor expression and release in allergic inflammatory disease of the upper airways. Am J Respir Crit Care Med 2000-05-01 [PMID: 10806167]
• Ullrich A, Gray A, Berman C, Dull TJ. Human beta-nerve growth factor gene sequence highly homologous to that of mouse. Nature. 1983-06-30 [PMID: 6688123]

FAQs for beta-NGF ELISA Kit (KA0401). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: NGF