beta-NGF Antibody (347112) [Unconjugated]

• Reactivity: Hu
• Applications: WB
• Clone: 347112
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

beta-NGF Antibody (347112) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human pro-beta -NGF
  Glu19-Ala241
  Accession # P01138
• Specificity: Detects human pro-beta -NGF in Western blots. In direct ELISAs and Western blots, this antibody shows no cross-reactivity with recombinant mouse (rm) beta -NGF, rr beta -NGF, rhBDNF, rhNT-3, or rhNT-4.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: NGF
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for beta-NGF Antibody (347112) [Unconjugated]

• Beta nerve growth factor precursor
• beta-nerve growth factor
• betaNGF
• beta-NGF
• HSAN5
• MGC161426
• nerve growth factor (beta polypeptide)
• nerve growth factor, beta subunit
• NGFB
• NGFBMGC161428

Background

NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha , beta , and gamma . Both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta -NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity. NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor-dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-NGF Antibody (MAB2562)(3)

Publications using MAB2562

• S Nyström, A Kendall, S Adepu, A Lindahl, E Skiöldebra The expression of nerve growth factor in healthy and inflamed equine chondrocytes analysed by capillary western immunoassay Research in Veterinary Science, 2022-08-22;151(0):156-163. 2022-08-22 [PMID: 36029605] (IHC, Horse)
• C Cremon, V Stanghelli, MR Barbaro, RF Cogliandro, L Bellacosa, J Santos, M Vicario, M Pigrau, C Alonso Cot, B Lobo, F Azpiroz, S Bruley des, M Neunlist, D DeFilippis, T Iuvone, S Petrosino, V Di Marzo, G Barbara Randomised clinical trial: the analgesic properties of dietary supplementation with palmitoylethanolamide and polydatin in irritable bowel syndrome Aliment. Pharmacol. Ther, 2017-02-06;0(0):. 2017-02-06 [PMID: 28164346] (Western Blot, Human)
• F Zeng, Z Li, Q Zhu, R Dong, C Zhao, G Li, G Li, W Gao, G Jiang, E Zheng, G Cai, S Moisyadi, J Urschitz, H Yang, D Liu, Z Wu Production of functional human nerve growth factor from the saliva of transgenic mice by using salivary glands as bioreactors Sci Rep, 2017-01-24;7(0):41270. 2017-01-24 [PMID: 28117418] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: NGF
• Uniprot:
  • P01138()