beta-NGF Antibody (25617) [Unconjugated]

Recombinant Human beta ‑NGF protein (256-GF) was serially diluted 2-fold and captured by Mouse Anti-Human beta ‑NGF Monoclonal Antibody (Catalog # MAB2561) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human beta ‑NGF Biotinylated Antigen Affinity-purified Polyclonal Antibody (BAF256) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

• Reactivity: Hu
• Applications: ELISA(Cap)
• Clone: 25617
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

beta-NGF Antibody (25617) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human beta -NGF
  Ser122-Ala241
  Accession # CAA36832
• Specificity: Detects human beta -NGF in direct ELISAs. In sandwich immunoassays, cross-reactivity with recombinant mouse (rm) beta ‑NGF and recombinant rat (rr) beta ‑NGF has not been determined. In direct ELISAs, 100% cross-reactivity with rm beta ‑NGF and rr beta ‑NGF is observed, and no cross-reactivity with recombinant human (rh) BDNF, rhNT-3, or rhNT-4 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NGF
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for beta-NGF Antibody (25617) [Unconjugated]

• Beta nerve growth factor precursor
• beta-nerve growth factor
• betaNGF
• beta-NGF
• HSAN5
• MGC161426
• nerve growth factor (beta polypeptide)
• nerve growth factor, beta subunit
• NGFB
• NGFBMGC161428

Background

NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha , beta , and gamma . Both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta -NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity.

NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor-dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-NGF Antibody (MAB2561)(5)

Publications using MAB2561

• Kai A. Kropp, Alberto Domingo López-Muñoz, Birgit Ritter, Rocío Martín, Alberto Rastrojo, Sangar Srivaratharajan et al. Herpes Simplex Virus 2 Counteracts Neurite Outgrowth Repulsion during Infection in a Nerve Growth Factor-Dependent Manner Journal of Virology 9/29/2020 [PMID: 32669337]
• N Zareen, S C Biswas, L A Greene A feed-forward loop involving Trib3, Akt and FoxO mediates death of NGF-deprived neurons Cell Death & Differentiation 12/1/2013 [PMID: 24212932]
• Pontus Forsell, Helena Almqvist, Per Hillertz, Tomas Åkerud, Magdalena Otrocka, Lina Eisele et al. The Use of TrkA-PathHunter Assay in High-Throughput Screening to Identify Compounds That Affect Nerve Growth Factor Signaling J Biomol Screen 7/1/2013 [PMID: 23458757]
• Chang EJ, Im YS, Kay EP The role of nerve growth factor in hyperosmolar stress induced apoptosis. J. Cell. Physiol., 2008-07-01;216(1):69-77. 2008-07-01 [PMID: 18300262] (IHC-P, Rat)
• Hayashi K, Storesund T, Schreurs O Nerve growth factor beta/pro-nerve growth factor and their receptors in normal human oral mucosa. Eur. J. Oral Sci., 2007-10-01;115(5):344-54. 2007-10-01 [PMID: 17850422] (Neutralization, Human)

Bioinformatics

• Gene Symbol: NGF
• Uniprot:
  • CAA36832()