beta-III Tubulin Antibody (EP1569Y)

Product Discontinued

beta-III Tubulin Antibody (EP1569Y) Summary

• Immunogen: A synthetic peptide corresponding to the C-term of human class III Beta-Tubulin was used as immunogen.
• Marker: Neuronal Marker
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TUBB3
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10
  • Immunocytochemistry/ Immunofluorescence 1:100-250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1:50-100
  • Immunoprecipitation 1:40
  • Western Blot 1:1000-10000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  beta-III Tubulin Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for beta-III Tubulin Antibody (EP1569Y)

• Beta3-tubulin
• beta-4
• betaIII Tubulin
• beta-III Tubulin
• beta-Tubulin III
• Class III beta-tubulin
• TUBB3
• TUBB4 CFEOM3A
• Tubulin Beta 3
• tubulin beta-3 chain
• Tubulin beta-4 chain
• Tubulin beta-4
• Tubulin beta-III
• tubulin, beta 3
• TUJ1 antigen
• TUJ-1 antigen

Background

Microtubules are essential parts in eukaryotic cell structures, transportation, and mitosis and consists mainly of 2 soluble protein subunits, alpha and beta tubulin. Beta-tubulin binds to alpha tubulin to form tubulin heterodimer which is post-translationally modified (1). The tubulin dimer complex binds to GTP and assembles onto the positive ends of microtubules. After incorporation into the microtubules, bound GTP is hydrolyzed by beta tubulin. The stability of the dimer in the microtubules is depended on presence of beta tubulin, where dimer with GTP bound beta-tubulin is stable to microtubule incorporation (2). Class III beta tubulin (beta III-tubulin) is a vertebrate tubulin Isotype specific to the neurons and mammalian testis cells, making it an ideal neuronal marker. Overexpression of class III beta tubulin is associated with the resistances of microtubule-targeted cancer drugs in lung cancer cell lines, breast cancer cell lines, and ovarian tumors (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-III Tubulin Antibody (NB110-57611)(6)

Publications using NB110-57611

• Eriksson I, Vainikka L, W�ster P, �llinger K Lysosomal function and intracellular position determine the malignant phenotype in malignant melanoma The Journal of investigative dermatology 2023-03-03 [PMID: 36871659] (Immunocytochemistry/ Immunofluorescence, Human)
• Mollinari C, De Dominicis C, Lupacchini L et al. Detection of Pathological Markers of Neurodegenerative Diseases following Microfluidic Direct Conversion of Patient Fibroblasts into Neurons International journal of molecular sciences 2022-02-15 [PMID: 35216271] (ICC/IF, Human)
• Inada, E;Saitoh, I;Kubota, N;Iwase, Y;Murakami, T;Sawami, T;Yamasaki, Y;Sato, M; Increased Expression of Cell Surface SSEA-1 is Closely Associated with Naive-Like Conversion from Human Deciduous Teeth Dental Pulp Cells-Derived iPS Cells Int J Mol Sci 2019-04-03 [PMID: 30987116] (ICC/IF, Human)
• Inada E, Saitoh I, Watanabe S et al. PiggyBac transposon-mediated gene delivery efficiently generates stable transfectants derived from cultured primary human deciduous tooth dental pulp cells (HDDPCs) and HDDPC-derived iPS cells. Int J Oral Sci 2015-09-14 [PMID: 26208039] (ICC/IF, Human)
• Wang YY, Wu HI, Hsu WL et al. In vitro growth conditions and development affect differential distributions of RNA in axonal growth cones and shafts of cultured rat hippocampal neurons Mol. Cell. Neurosci. 2014-06-28 [PMID: 24983517] (ICC/IF, Rat)
  
  Details:
  Tubulin Beta 3 antibody used for ICC-IF on cultured rat hippocampal neurons, fixed in 4% PFA for 30 min, permeabilized with 0.05% Triton X-100 -PBS for 15 min, 1:100 primary dilution - incubation for 2h at 37C (Fig. 2B )
• Imamura T, Ishizuka O, Lei Z, Hida S, Sudha GS, Kato H, Nishizawa O. Bone Marrow-Derived Cells Implanted into Radiation-Injured Urinary Bladders Reconstruct Functional Bladder Tissues in Rats. Tissue Eng Part A. 2012-05-14 [PMID: 22480258] (IF/IHC, ICC/IF, Rat)

Bioinformatics

• Gene Symbol: TUBB3
• Entrez:
  • 10381(Human)
  • 22152(Mouse)
  • 246118(Rat)
• Uniprot:
  • Q13509(Human)