beta II Tubulin A Antibody

Western Blot: beta II Tubulin A Antibody [NBP2-15580] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:5000.

Immunocytochemistry/Immunofluorescence: beta II Tubulin A Antibody [NBP2-15580] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: beta II Tubulin A Antibody [NBP2-15580] - Paraffin-embedded colon carcinoma. beta Tubulin 2 antibody [N1C1] dilution: 1:500.

Western Blot: beta II Tubulin A Antibody [NBP2-15580] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE gel, diluted at 1:3000.

Western Blot: beta II Tubulin A Antibody [NBP2-15580] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:1000.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

beta II Tubulin A Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human beta Tubulin 2. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TUBB2A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for beta II Tubulin A Antibody

• beta II Tubulin A
• dJ40E16.7
• TUBB
• TUBB2
• TUBB2A
• TUBB2B
• tubulin beta-2A chain
• tubulin, beta 2
• tubulin, beta 2A
• tubulin, beta polypeptide 2
• tubulin, beta polypeptide

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: TUBB2A
• Uniprot:
  • Q13885(Human)