beta I Tubulin Antibody (923425) [Unconjugated]

Images

 
Western blot shows lysates of human platelets and mouse platelets. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human beta -I Tubulin Monoclonal Antibody (Catalog # MAB8527) followed by HRP-conjugated ...read more
beta -I Tubulin was detected in immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes using Mouse Anti-Human beta -I Tubulin Monoclonal Antibody (Catalog # MAB8527) at 10 µg/mL for 3 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
923425
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

beta I Tubulin Antibody (923425) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human beta -I Tubulin
Met1-His451
Accession # Q9H4B7
Specificity
Detects human beta -I Tubulin in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
TUBB1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publication using
MAB8527 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for beta I Tubulin Antibody (923425) [Unconjugated]

  • beta tubulin 1, class VI
  • betaI Tubulin
  • beta-I Tubulin
  • dJ543J19.4
  • TUBB1
  • tubulin beta-1 chain
  • tubulin, beta 1

Background

beta -tubulin is a 50 kDa cytoskeletal protein which is the a major component of neuronal processes: axons, dendrites, and dendritic spines. beta -tubulin is represented by different isoforms including beta -I, beta -II, beta -III, and beta -IV. Elimination of beta -I isoform will decrease the viability of neuroblastoma cells. Formation of actin tubules may be directly inhibited by beta -I isoform which results in decreasing cellular adhesion. beta -I Tubulin levels in different brain regions of schizophrenia patients  undergo noticeable changes suggesting that this isoform may have a specific role in the pathophysiology of this illness by affecting actin tubule formation which is required for cell division and differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for beta I Tubulin Antibody (MAB8527)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Product General Protocols

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Secondary Antibodies

 

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Bioinformatics Tool for beta I Tubulin Antibody (MAB8527)

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Bioinformatics

Gene Symbol TUBB1
Uniprot