beta-Defensin 3 Antibody


Immunohistochemistry-Paraffin: Defensin beta 3 Antibody [NB200-117] - IHC analysis of Defensin beta 3 in human skin.

Product Details

Reactivity Hu, Rt, Canine, PorcineSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
1 mg/ml

Order Details

beta-Defensin 3 Antibody Summary

A synthetic peptide made to an internal portion of Defensin beta 3 (between amino acids 10-50) [UniProt P81534]
Predicted Species
Porcine (90%), Canine (90%). Backed by our 100% Guarantee.
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1 mg/ml
Immunogen affinity purified


  • Western Blot 1:500-1:1000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Immunocytochemistry/Immunofluorescence was reported in scientific literature.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
7.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NB200-117 in the following applications:

Reactivity Notes

Human and rat (PMID: 21369366).

Alternate Names for beta-Defensin 3 Antibody

  • BD14
  • BD3
  • BD-3
  • beta-defensin 103
  • betaDefensin 3
  • Beta-defensin 3
  • DEFB103
  • DEFB103A
  • DEFB103B
  • DEFB3
  • DEFB-3
  • Defensin, beta 103
  • defensin, beta 103B
  • Defensin-like protein
  • hBD-3
  • HBD3HBP3


A novel, nonhemolytic antimicrobial peptide (human beta-defensin-3, hBD-3) was isolated from human lesional psoriatic scales and clones from keratinocytes. hBD-3 demonstrated a salt-insensitive broad spectrum of potent antimicrobial activity against many potentially pathogenic microbes including multiresistant S. aureus and vancomyosin-resistant Entoerococcus faecium. Keratinocytes and airway epithelial cells are cellular sources of hBD-3. Tumor necrosis factor alpha and contact with bacteria were found to induce hBD-3 mRNA expression. hBD-3, therefore, might be important in the innate epithelial defense of infections by various microorganisms seen in skin and lung, such as cystic fibrosis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for beta-Defensin 3 Antibody (NB200-117)(13)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.

Filter By Application
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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB200-117 Applications Species
Koerdt S, Steinstraesser L, Stoeckelhuber M. Radiotherapy for oral cancer decreases the cutaneous expression of host defence peptides. J Craniomaxillofac Surg. Apr 22 2016 12:00AM [PMID:27193476] (IHC-P, Human) IHC-P Human
Jeong KY, Kim HM. Neonatal capsaicin treatment in rats induces chronic hyperthermia resulting in infectious disease. EXPERIMENTAL AND THERAPEUTIC MEDICINE. 2015 Sep 01 [PMID:26668650] (WB, Rat) WB Rat
Qian YJ, Wang X, Gao YF et al. Cigarette Smoke Modulates NOD1 Signal Pathway and Human B Defensins Expression in Human Oral Mucosa. Cell. Physiol. Biochem. 2015 May 29 [PMID:25968832] (ICC/IF, IHC-P, Human) ICC/IF, IHC-P Human
Levon J, Al-Samadi A, Mackiewicz Z et al. Human beta-defensin-3 producing cells in septic implant loosening J Mater Sci Mater Med. 2015 Feb 01 [PMID:25655501] (IHC-P, Human) IHC-P Human
Simon D, Radonjic-Hosli S, Straumann A et al. Active eosinophilic esophagitis is characterized by epithelial barrier defects and eosinophil extracellular trap formation Allergy. 2015 Jan 05 [PMID:25620273] (IHC-P, Human) IHC-P Human
Stoeckelhuber M, Olzowy B, Ihler F et al. Immunolocalization of antimicrobial and cytoskeletal components in the serous glands of human sinonasal mucosa. Histol. Histopathol. 2014 Apr 15 [PMID:24737387] (IHC-P, Human) IHC-P Human
Zhu C, Wang J, Cheng T et al. The Potential Role of Increasing the Release of Mouse beta-Defensin-14 in the Treatment of Osteomyelitis in Mice: A Primary Study. PLoS ONE 2014 Feb 3 [PMID:24489798] (IHC-P, WB, Human) IHC-P, WB Human
Guttsches AK, Jacobsen F, Theiss C et al. Human beta-defensin-3 correlates with muscle fibre degeneration in idiopathic inflammatory myopathies. Innate Immun 2013 Apr 22 [PMID:23608825] (IHC-Fr, ICC/IF, Human) IHC-Fr, ICC/IF Human
Boldenow E, Jones S, Lieberman RW et al. Antimicrobial peptide response to Group B Streptococcus in human extraplacental membranes in culture Placenta 2013 Apr 2 [PMID:23562109] (IHC-P, Human) IHC-P Human
Weinberg A, Jin G, Sieg S, McCormick TS. The Yin and Yang of Human Beta-Defensins in Health and Disease Front Immunol 2012 [PMID:23060878] (IHC, ICC/IF, Human) IHC, ICC/IF Human
Show All 13 Publications.

Reviews for beta-Defensin 3 Antibody (NB200-117) (0)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta-Defensin 3 Antibody (NB200-117). (Showing 1 - 1 of 1 FAQs).

  1. Are there by chance any WB images available for NB200-117 that haven’t been posted yet? If not, do you happen to know what band size(s) were actually detected? We have a customer who is concerned because he has encountered several products that are detecting around 15-17 kDa as opposed to the expected mw of ~8 kDa.
    • We unfortunately do not have any images available for this product in western blot. I am sorry for any inconvenience. Since western blot is listed on our datasheet, we will of course fully guarantee the application. Typically a band is seen around 10kDa. Depending on the molecular weight marker, I would expect that there might be some difference seen at such a low molecular weight. 15-17kDa does seem a bit too high. I noticed that the protein does have several disulfide binding points. I would make sure your customer is properly boiling their samples (we recommend 10 minutes at 95C) and that they are adding at least 5% reducing agent to their sample buffer. We use BME typically, but DTT is also good.

Secondary Antibodies


Isotype Controls

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Blogs on beta-Defensin 3.

Beta-defensin-3: I may be small but I'm powerful!
Beta-defensin 3 is a novel, non-hemolytic antimicrobial cationic peptide originally isolated from human lesional psoriatic scales and keratinocyte clones. It is a very small (2-6 kD) yet potent salt-insensitive broad spectrum antimicrobial that ta...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol DEFB103B

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