Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Immunogen affinity purified
Western Blot 1:500-1:1000
Immunocytochemistry/Immunofluorescence was reported in scientific literature.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
7.7 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
A novel, nonhemolytic antimicrobial peptide (human beta-defensin-3, hBD-3) was isolated from human lesional psoriatic scales and clones from keratinocytes. hBD-3 demonstrated a salt-insensitive broad spectrum of potent antimicrobial activity against many potentially pathogenic microbes including multiresistant S. aureus and vancomyosin-resistant Entoerococcus faecium. Keratinocytes and airway epithelial cells are cellular sources of hBD-3. Tumor necrosis factor alpha and contact with bacteria were found to induce hBD-3 mRNA expression. hBD-3, therefore, might be important in the innate epithelial defense of infections by various microorganisms seen in skin and lung, such as cystic fibrosis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Muller C. Quantitative studies of the differential expression of effector molecules of the innate immune system in between oral mucosa and extraoral skin. Thesis: http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:91-diss-20140205-1128930-0-1 2014 Feb 05 (IHC-P, Human)
FAQs for beta-Defensin 3 Antibody (NB200-117). (Showing 1 - 1 of 1 FAQs).
Are there by chance any WB images available for NB200-117 that haven’t been posted yet? If not, do you happen to know what band size(s) were actually detected? We have a customer who is concerned because he has encountered several products that are detecting around 15-17 kDa as opposed to the expected mw of ~8 kDa.
We unfortunately do not have any images available for this product in western blot. I am sorry for any inconvenience. Since western blot is listed on our datasheet, we will of course fully guarantee the application. Typically a band is seen around 10kDa. Depending on the molecular weight marker, I would expect that there might be some difference seen at such a low molecular weight. 15-17kDa does seem a bit too high. I noticed that the protein does have several disulfide binding points. I would make sure your customer is properly boiling their samples (we recommend 10 minutes at 95C) and that they are adding at least 5% reducing agent to their sample buffer. We use BME typically, but DTT is also good.