beta-Catenin Antibody

Product Discontinued

beta-Catenin Antibody Summary

• Immunogen: The antiserum was produced against synthesized peptide derived from human Catenin-beta. The immunogen region is: C-terminal.
• Marker: Epithelial Cell Marker, Adherens Junction Marker
• Specificity: Catenin-beta (Ab-654) Antibody detects endogenous levels of total Catenin-beta protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CTNNB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:40000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Western Blot 1:500-1:1000

Reactivity Notes

Human,Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+, pH 7.4), 0.15M NaCl and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for beta-Catenin Antibody

• bCatenin
• beta 1 (88kD)
• beta-Catenin
• catenin (cadherin-associated protein), beta 1, 88kDa
• catenin beta-1
• CTNNB
• CTNNB1
• DKFZp686D02253
• FLJ25606
• FLJ37923

Background

The catenins, a, beta and ', are proteins that bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherin complexes play critical roles in mediating cellular adhesion. beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. beta-catenin also forms complexes with the tumor suppressor protein APC. Amino acid alterations at residues around Ser 33, one of the targets for phosphorylation of glycogen synthase kinase-3beta, result in accumulation of the beta-catenin protein in the cytoplasm and nucleus. Pin1 is a novel regulator of beta-catenin signaling that directly binds a phosphorylated Ser-Pro motif next to the APC-binding site in beta-catenin, inhibiting the interaction with APC and increasing beta-catenin translocation into the nucleus. Thus, Pin1 overexpression may contribute to the upregulation of beta-catenin in tumors such as breast cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CTNNB1
• Uniprot:
  • P35222()