beta-Catenin Antibody (E247)

Product Discontinued

beta-Catenin Antibody (E247) Summary

• Immunogen: A synthetic peptide corresponding to residues near N-terminus of human Beta-catenin was used as immunogen.
• Marker: Epithelial Cell Marker, Adherens Junction Marker
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CTNNB1
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:250
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:500
  • Immunoprecipitation 1:100
  • Western Blot 1:5000-10000
• Application Notes: This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation. In Western blot this antibody detects a band at approximately 92kDa.
• Theoretical MW: 86 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  beta-Catenin Overexpression Lysate

Reactivity Notes

Reacts with: Macaque Monkey, African Green Monkey

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for beta-Catenin Antibody (E247)

• bCatenin
• beta 1 (88kD)
• beta-Catenin
• catenin (cadherin-associated protein), beta 1, 88kDa
• catenin beta-1
• CTNNB
• CTNNB1
• DKFZp686D02253
• FLJ25606
• FLJ37923

Background

The protein encoded by the beta Catenin gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs arenecessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion betweencells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contactinhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this proteinbinds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this geneare a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-Catenin Antibody (NB110-56924)(4)

Publications using NB110-56924

• Uzawa Katsuhiro, Kasamatsu Atsushi, Shimizu Toshihiro et al. Suppression of metastasis by mirtazapine via restoration of the Lin-7C/beta-catenin pathway in human cancer cells. Sci Rep. 2014-06-25 [PMID: 24961284] (Human)
• Li X, Gonzalez ME, Toy K et al. Targeted Overexpression of EZH2 in the Mammary Gland Disrupts Ductal Morphogenesis and Causes Epithelial Hyperplasia ,Am. Am. J. Pathol. 175 1246 - 1254. 2009-01-01 [PMID: 19661437] (IHC)
• Gaspar C, Franken P, Molenaar L et al. A Targeted Constitutive Mutation in the Apc Tumor Suppressor Gene Underlies Mammary But Not Intestinal Tumorigenesis . PLoS Genet 5 e1000547. 2009-01-01 [PMID: 19578404] (IHC)
• van Dekken H, Wink JC, Vissers KJ et al. Wnt pathway-related gene expression during malignant progression in ulcerative colitis. Acta Histochem 2007-01-01 [PMID: 17445872] (IHC)

Bioinformatics

• Gene Symbol: CTNNB1
• Entrez:
  • 1499(Human)
  • 12387(Mouse)
  • 84353(Rat)
• Uniprot:
  • P35222()