Immunohistochemistry-Paraffin: beta Amyloid Antibody [NBP2-25093] - Human brain (Alzheimer's disease) tissue with APP antibody at 10 ug/mL.
Immunohistochemistry: beta Amyloid Antibody [NBP2-25093] - Rat heart tissue with ASAH1 antibody at 20 ug/mL.Green: APP Antibody Blue: DAPI staining
Western Blot: beta Amyloid Antibody [NBP2-25093] - human (A), mouse (B), and rat (C) brain tissue lysates with APP antibody at 1 ug/mL. Note: observed band is higher than predicted molecular weight of 5 kDa.
Immunohistochemistry-Paraffin: beta Amyloid Antibody [NBP2-25093] - Analysis of APP in human brain tissue with APP antibody at 2.5 ug/ml.
This beta Amyloid antibody was raised against a 10 amino acid peptide on the amino terminus of the 4KDa beta Amyloid peptide generated by beta- and gamma-secretases. The immunogen is located within amino acids 650 - 700 of human amyloid A4 protein precursor (APP). Amino Acid Squence: DAEFRHDSGYE
Use in IP reported in secitific publication PMID: 32413239
115 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 3 using NBP2-25093 in the following applications:
Use in Pseudomonas aeruginosa reported in secitific publication PMID: 32413239
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Peptide affinity purified
Alternate Names for beta Amyloid Antibody
Beta amyloid, also known as Abeta, is a peptide that varies in length from 39 to 43 amino acids and is processed from the transmembrane protein amyloid precursor protein (APP) by beta-secretase (BACE-1) and gamma-secretase. Beta amyloid [1-40], beta amyloid [1-42], and beta amyloid [1-43] peptides result from cleavage of APP after residues 40, 42, and 43, respectively. The aggregation of beta amyloid monomers produces insoluble oligomers and protofibrils. The longer "stickier" forms of beta amyloid, particularly ABeta42, are known as the main component of amyloid plaques, which are deposits (senile plaques or neuritic plaques) found in the brains of patients with Alzheimer's disease (AD) (1,2). Research indicates that intraneuronal beta amyloid accumulation may be an important proximal neurotoxic event in the pathogenesis of Alzheimer's disease. Abeta deposits have also been reported in the hearts of AD patients (3).
Pyroglutamate amyloid beta peptides (pGlu-Abeta) are N-terminal truncations in which the N-terminal glutamate is cyclized to pyroglutamate resulting in pGlu-Abeta (3-40/42 and 11-40/42) (4). This pyrE modification may have a greater propensity to aggregate under physiological conditions and has been implicated as the molecular species responsible for seeding larger oligomers of amyloid beta.
1. Chen GF, Xu TH, Yan Y, Zhou YR, Jiang Y, Melcher K, Xu HE. (2017) Amyloid beta: structure, biology and structure-based therapeutic development. Acta Pharmacol Sin. 38(9):1205-1235. PMID: 28713158
3. Schaich CL, Maurer MS, Nadkarni NK. (2019) Amyloidosis of the Brain and Heart: Two Sides of the Same Coin? JACC Heart Fail. 7(2):129-131. PMID: 30704604
4. He W, Barrow CJ. (1999) The A beta 3-pyroglutamyl and 11-pyroglutamyl peptides found in senile plaque have greater beta-sheet forming and aggregation propensities in vitro than full-length A beta. Biochemistry. 38(33):10871-7. PMID: 10451383
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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