beta 2-Microglobulin Antibody (BBM.1) - Chimeric - Azide and BSA Free

Western Blot: beta 2-Microglobulin Antibody (BBM.1) - Chimeric [NBP2-81045] - Western Blot using anti-Beta-2 microglobulin antibody BBM.1 [NBP2-81045]. HeLa cell lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of BBM.1 [NBP2-81045] at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Beta-2 microglobulin is 13.7 kDa. NBP2-81045 successfully detected human Beta-2 microglobulin in both HeLa cell extract and human duodenum samples.

Flow Cytometry: beta 2-Microglobulin Antibody (BBM.1) - Chimeric [NBP2-81045] - Flow-cytometry using the anti-Beta-2 microglobulin antibody BBM.1 [NBP2-81045]. HeLa cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of BBM.1 (NBP2-81045, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.

Immunocytochemistry /Immunofluorescence: beta 2-Microglobulin Antibody (BBM.1) - Chimeric - Azide and BSA Free [NBP2-81045] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with NBP2-81045 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP2-81045, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: BBM.1
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

beta 2-Microglobulin Antibody (BBM.1) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit beta 2-Microglobulin Antibody (BBM.1) - Chimeric - Azide and BSA Free (NBP2-81045) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Molt 4, a human T cell line.
• Specificity: Binds human beta 2-Microglobulin, a component of MHC class I molecules, which are present on all nucleated cells (excludes red blood cells) and involved in the presentation of peptide antigens to the immune system.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: B2M
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for beta 2-Microglobulin Antibody (BBM.1) - Chimeric - Azide and BSA Free

• B2M
• beta 2Microglobulin
• beta 2-Microglobulin
• beta chain of MHC class I molecules
• beta-2-microglobin
• beta-2-microglobulin

Background

Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells (Gussow et al., 1987 [PubMed 3312414]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: B2M