beta 2-Microglobulin Antibody (246-E9.E7 (same as HLA.ABC.m2)) - Azide and BSA Free

• Reactivity: Hu, Pm(-)
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: 246-E9.E7 (same as HLA.ABC.m2)
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

beta 2-Microglobulin Antibody (246-E9.E7 (same as HLA.ABC.m2)) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44465).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Human PBL s from a T-cell acute lymphoblastic leukemia (T-ALL) patient
• Localization: Cell Surface and Extracellular (Secreted)
• Marker: Renal Failure & Tumor Marker
• Specificity: Recognizes a protein of 12kDa, identified as beta-microglobulin. Beta--microglobulin non-covalently associates with the 44kDa chain to form the HLA Class I antigen complex. Human beta-2 microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes. This monoclonal antibody is specific to human beta-2 microglobulin and does not react with non-human primate cells. This antibody reacts with all cell types excluding erythrocytes. Detection of beta-2 microglobulin in body fluids has been used as a tumor marker and for monitoring patients with HIV infection.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: B2M
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
• Application Notes: Optimal dilution for a specific application should be determined.
• Theoretical MW: 12 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Does not react with Non-Human primates.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for beta 2-Microglobulin Antibody (246-E9.E7 (same as HLA.ABC.m2)) - Azide and BSA Free

• B2M
• beta 2Microglobulin
• beta 2-Microglobulin
• beta chain of MHC class I molecules
• beta-2-microglobin
• beta-2-microglobulin

Background

Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells (Gussow et al., 1987 [PubMed 3312414]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: B2M