beta 2-Microglobulin Antibody (246-E9.E7 (same as HLA.ABC.m2)) [CoraFluor™ 1]

• Reactivity: Hu, Pm(-)
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: 246-E9.E7 (same as HLA.ABC.m2)
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

beta 2-Microglobulin Antibody (246-E9.E7 (same as HLA.ABC.m2)) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
• Immunogen: Human PBL s from a T-cell acute lymphoblastic leukemia (T-ALL) patient
• Localization: Cell Surface and Extracellular (Secreted)
• Marker: Renal Failure & Tumor Marker
• Specificity: Recognizes a protein of 12kDa, identified as beta-microglobulin. Beta--microglobulin non-covalently associates with the 44kDa chain to form the HLA Class I antigen complex. Human beta-2 microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes. This monoclonal antibody is specific to human beta-2 microglobulin and does not react with non-human primate cells. This antibody reacts with all cell types excluding erythrocytes. Detection of beta-2 microglobulin in body fluids has been used as a tumor marker and for monitoring patients with HIV infection.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: B2M
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunofluorescence
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Does not react with Non-Human primates.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for beta 2-Microglobulin Antibody (246-E9.E7 (same as HLA.ABC.m2)) [CoraFluor™ 1]

• B2M
• beta 2Microglobulin
• beta 2-Microglobulin
• beta chain of MHC class I molecules
• beta-2-microglobin
• beta-2-microglobulin

Background

Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells (Gussow et al., 1987 [PubMed 3312414]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: B2M