beta 2-Microglobulin Antibody (246-E9.E7 (same as HLA.ABC.m2)) [PE/Atto594]

• Reactivity: Hu, Pm(-)
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: 246-E9.E7 (same as HLA.ABC.m2)
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PE/Atto594

beta 2-Microglobulin Antibody (246-E9.E7 (same as HLA.ABC.m2)) [PE/Atto594] Summary

• Immunogen: Human PBL s from a T-cell acute lymphoblastic leukemia (T-ALL) patient
• Localization: Cell Surface and Extracellular (Secreted)
• Marker: Renal Failure & Tumor Marker
• Specificity: Recognizes a protein of 12kDa, identified as beta-microglobulin. Beta--microglobulin non-covalently associates with the 44kDa chain to form the HLA Class I antigen complex. Human beta-2 microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes. This monoclonal antibody is specific to human beta-2 microglobulin and does not react with non-human primate cells. This antibody reacts with all cell types excluding erythrocytes. Detection of beta-2 microglobulin in body fluids has been used as a tumor marker and for monitoring patients with HIV infection.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: B2M
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunofluorescence
• Application Notes: Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Reactivity Notes

Does not react with Non-Human primates.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for beta 2-Microglobulin Antibody (246-E9.E7 (same as HLA.ABC.m2)) [PE/Atto594]

• B2M
• beta 2Microglobulin
• beta 2-Microglobulin
• beta chain of MHC class I molecules
• beta-2-microglobin
• beta-2-microglobulin

Background

Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells (Gussow et al., 1987 [PubMed 3312414]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: B2M