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![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: beta-1 Adrenergic R/ADRB1 Antibody [NB600-978] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/beta-1-Adrenergic-R-ADRB1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-978-img0003.jpg)
![Immunohistochemistry-Paraffin: beta-1 Adrenergic R/ADRB1 Antibody [NB600-978] - Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.](http://images.novusbio.com/fullsize/beta-1-Adrenergic-R-ADRB1-Antibody-Immunohistochemistry-Paraffin-NB600-978-img0002.jpg)
![Immunohistochemistry-Paraffin: beta-1 Adrenergic R/ADRB1 Antibody [NB600-978] - Staining of paraffin embedded Human Heart with antibody at 6 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/beta-1-Adrenergic-R-ADRB1-Antibody-Immunohistochemistry-Paraffin-NB600-978-img0001.jpg)
![Western Blot: beta-1 Adrenergic R/ADRB1 Antibody [NB600-978] - Effects of MPP Versus PHTPP on NE Regulation of VMN Nitrergic & GABA Neuron Adrenergic Receptor Protein Expression. Pooled lysates of laser-microdissected VMN nNOS- or GAD-immunopositive neurons from groups of female rats pretreated with V versus ER alpha or -beta antagonist prior to intra-VMN V or NE infusion were analyzed by Western blot for alpha1- ( alpha 1-), alpha2- ( alpha 2-), or beta1- ( beta 1-) AR protein expression. Nitrergic neuron alpha 1-, F(5, 12) = 10.51, p = .0005; alpha 2-, F(5, 12) = 16.50, p < .0001; & beta 1-, F(5, 12) = 11.72, p = .0003 protein profiles are depicted in Panels 3A to C; GABAergic neuron alpha 1-, F(5, 12) = 5.52, p = .007; alpha 2-, F(5, 12) = 10.47, p < .0001; & beta 1-, F(5, 12) = 12.21, p = .0002 protein profiles are presented in Panels 3D to F. Data show mean normalized protein O.D. measures ± SEM for the following treatment groups: Veh/Veh (solid white bars, n = 6), MPP/Veh (diagonal-striped white bars, n = 6), PHTPP/Veh (cross-hatched white bars, n = 6), Veh/NE (solid gray bars, n = 6), MPP/NE (diagonal-striped gray bars, n = 6), & PHTPP/NE (cross-hatched gray bars, n = 6). *p < .05; **p < .01; ***p < .001. alpha 1-AR = alpha1 adrenergic receptor; alpha 2-AR = alpha2 adrenergic receptor; beta 1-AR = beta1 adrenergic receptor; MPP = 1,3-Bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazole dihydrochloride; PHTPP = 4-[2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl]phenol; NE = norepinephrine. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32233668), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-978_goat-polyclonal-beta-1-adrenergic-r-adrb1-antibody-310202416163766.jpg)
![Western Blot: beta-1 Adrenergic R/ADRB1 Antibody [NB600-978] - Effects of MPP Versus PHTPP on NE Regulation of VMN Nitrergic & GABA Neuron Adrenergic Receptor Protein Expression. Pooled lysates of laser-microdissected VMN nNOS- or GAD-immunopositive neurons from groups of female rats pretreated with V versus ER alpha or -beta antagonist prior to intra-VMN V or NE infusion were analyzed by Western blot for alpha1- ( alpha 1-), alpha2- ( alpha 2-), or beta1- ( beta 1-) AR protein expression. Nitrergic neuron alpha 1-, F(5, 12) = 10.51, p = .0005; alpha 2-, F(5, 12) = 16.50, p < .0001; & beta 1-, F(5, 12) = 11.72, p = .0003 protein profiles are depicted in Panels 3A to C; GABAergic neuron alpha 1-, F(5, 12) = 5.52, p = .007; alpha 2-, F(5, 12) = 10.47, p < .0001; & beta 1-, F(5, 12) = 12.21, p = .0002 protein profiles are presented in Panels 3D to F. Data show mean normalized protein O.D. measures ± SEM for the following treatment groups: Veh/Veh (solid white bars, n = 6), MPP/Veh (diagonal-striped white bars, n = 6), PHTPP/Veh (cross-hatched white bars, n = 6), Veh/NE (solid gray bars, n = 6), MPP/NE (diagonal-striped gray bars, n = 6), & PHTPP/NE (cross-hatched gray bars, n = 6). *p < .05; **p < .01; ***p < .001. alpha 1-AR = alpha1 adrenergic receptor; alpha 2-AR = alpha2 adrenergic receptor; beta 1-AR = beta1 adrenergic receptor; MPP = 1,3-Bis(4-hydroxyphenyl)-4-methyl-5-[4-(2-piperidinylethoxy)phenol]-1H-pyrazole dihydrochloride; PHTPP = 4-[2-phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl]phenol; NE = norepinephrine. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32233668), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-978_goat-polyclonal-beta-1-adrenergic-r-adrb1-antibody-31020241616042.jpg)
![Western Blot: beta-1 Adrenergic R/ADRB1 Antibody [NBP1-59007] - Positive Control: Lane 1: 20ug Wild type mouse, left ventricle Lane 2: 20ug Transgenic mouse, treated with experimental drug , left ventricle Lane 3: 20ug HepG2 lysate](http://images.novusbio.com/fullsize/beta-1-Adrenergic-R-ADRB1-Antibody-Western-Blot-NBP1-59007-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: beta-1 Adrenergic R/ADRB1 Antibody [NBP1-59007] - Positive Control: Lane 1: 20ug Wild type mouse, left ventricle Lane 2: 20ug Transgenic mouse, treated with experimental drug , left ventricle Lane 3: 20ug HepG2 lysate")
![<b>Biological Strategies Validation. </b>Western Blot: beta-1 Adrenergic R/ADRB1 Antibody [NBP1-59007] - Titration: 0.2-1 ug/ml, Positive Control: Human brain.](http://images.novusbio.com/fullsize/beta-1-Adrenergic-R-ADRB1-Antibody-Western-Blot-NBP1-59007-img0003.jpg)
![Western Blot: beta-1 Adrenergic R/ADRB1 Antibody [NBP1-59007] - Human Fetal Heart Antibody Dilution: 1.0ug/ml.](http://images.novusbio.com/fullsize/beta-1-Adrenergic-R-ADRB1-Antibody-Western-Blot-NBP1-59007-img0004.jpg)
![Western Blot: beta-1 Adrenergic R/ADRB1 Antibody [NBP1-59007] - Human Fetal Lung Antibody Dilution: 1.0ug/m.](http://images.novusbio.com/fullsize/beta-1-Adrenergic-R-ADRB1-Antibody-Western-Blot-NBP1-59007-img0005.jpg)
![Western Blot: beta-1 Adrenergic R/ADRB1 Antibody [NBP1-59007] - Human Fetal Muscle Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/beta-1-Adrenergic-R-ADRB1-Antibody-Western-Blot-NBP1-59007-img0006.jpg)
![Western Blot: beta-1 Adrenergic R/ADRB1 Antibody [NBP1-59007] - Human Adult Placenta Antibody Dilution: 1.0ug/ml.](http://images.novusbio.com/fullsize/beta-1-Adrenergic-R-ADRB1-Antibody-Western-Blot-NBP1-59007-img0007.jpg)
![ELISA: Rat beta-1 Adrenergic R/ADRB1 ELISA Kit (Colorimetric) [NBP2-66654] - Samples were spiked with high concentrations of Rat beta-1 Adrenergic R/ADRB1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-66654_rat-beta-1-adrenergic-r-adrb1-elisa-kit-colorimetric-132202416204076.jpg "ELISA: Rat beta-1 Adrenergic R/ADRB1 ELISA Kit (Colorimetric) [NBP2-66654] - Samples were spiked with high concentrations of Rat beta-1 Adrenergic R/ADRB1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat beta-1 Adrenergic R/ADRB1 ELISA Kit (Colorimetric) [NBP2-66654] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-66654_rat-beta-1-adrenergic-r-adrb1-elisa-kit-colorimetric-432024983511.jpg)
