GNB3 Antibody

Images

 
Western Blot: GNB3 Antibody [NBP1-52107] - Analysis of GNB3 in HepG2 lysate (35ug protein in RIPA buffer) using this antibody at 0.1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: GNB3 Antibody [NBP1-52107] - (3.8ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

GNB3 Antibody Summary

Immunogen
Peptide with sequence SGHDNRVSCLGVT corresponding to internal region according to NP_002066.1, NP_067642.1.
Epitope
SGHDNRVSCLGVT
Specificity
This antibody is expected to recognize GNB3 & GNB4 (GeneID: 2784, 59345; ProteinID: NP_002066.1; NP_067642.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GNB3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 35 kDa band observed in HepG2 lysates (calculated MW of 37.2 kDa band according to NP_002066.1. IHC-P: Human cerebellum shows strong axonal staining in the molecular layer and less so in the granular layer.
Publications
Read Publication using NBP1-52107.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GNB3 Antibody

  • G protein, beta-3 subunit
  • GTP-binding regulatory protein beta-3 chain
  • guanine nucleotide binding protein (G protein), beta polypeptide 3
  • guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 3
  • guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3
  • hypertension associated protein
  • Transducin beta chain 3

Background

Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. A single-nucleotide polymorphism (C825T) in this gene is associated with essential hypertension and obesity. This polymorphism is also associated with the occurrence of the splice variant GNB3-s, which appears to have increased activity. GNB3-s is an example of alternative splicing caused by a nucleotide change outside of the splice donor and acceptor sites. Additional splice variants may exist for this gene, but they have not been fully described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GNB3 Antibody (NBP1-52107)(1)

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol GNB3
Entrez
Uniprot