GNB3 Antibody

Western Blot: GNB3 Antibody [NBP1-52107] - Analysis of GNB3 in HepG2 lysate (35ug protein in RIPA buffer) using this antibody at 0.1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: GNB3 Antibody [NBP1-52107] - (3.8ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

GNB3 Antibody Summary

• Description: Novus Biologicals Goat GNB3 Antibody (NBP1-52107) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-GNB3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence SGHDNRVSCLGVT corresponding to internal region according to NP_002066.1, NP_067642.1.
• Epitope: SGHDNRVSCLGVT
• Specificity: This antibody is expected to recognize GNB3 & GNB4 (GeneID: 2784, 59345; ProteinID: NP_002066.1; NP_067642.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GNB3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 35 kDa band observed in HepG2 lysates (calculated MW of 37.2 kDa band according to NP_002066.1. IHC-P: Human cerebellum shows strong axonal staining in the molecular layer and less so in the granular layer.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for GNB3 Antibody

• G protein, beta-3 subunit
• GTP-binding regulatory protein beta-3 chain
• guanine nucleotide binding protein (G protein), beta polypeptide 3
• guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 3
• guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3
• hypertension associated protein
• Transducin beta chain 3

Background

Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. A single-nucleotide polymorphism (C825T) in this gene is associated with essential hypertension and obesity. This polymorphism is also associated with the occurrence of the splice variant GNB3-s, which appears to have increased activity. GNB3-s is an example of alternative splicing caused by a nucleotide change outside of the splice donor and acceptor sites. Additional splice variants may exist for this gene, but they have not been fully described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GNB3 Antibody (NBP1-52107)(1)

Publication using NBP1-52107

• Gentile G, Missori S, Borro M et al. Frequencies of genetic polymorphisms related to triptans metabolism in chronic migraine. J Headache Pain 2010-04-01 [PMID: 20213484]

Bioinformatics

• Gene Symbol: GNB3
• Entrez:
  • 60449(Rat)
• Uniprot:
  • NP_002066.1(Human)