BCL11B Antibody - BSA Free

Western Blot: BCL11B Antibody [NB600-264] - Detection of Human Bcl11b by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from Jurkat and (50, 15 ug) MOLT-4 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Bcl11b antibody NB600-264 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry: BCL11B Antibody [NB600-264] - Detection of human Bcl11b by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Bcl11b (NB600-264). Detection: DAB

Immunoprecipitation: BCL11B Antibody [NB600-264] - Detection of Human Bcl11b on HeLa whole cell lysate using NB600-264. Bcl11b was also immunoprecipitated using affinity purified rabbit anti-Bcl11b antibodies NB600-262 and NB600-263.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

BCL11B Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit BCL11B Antibody - BSA Free (NB600-264) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-BCL11B Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide which maps to a region between residues 850 and the C-terminus (residue 894) of human B-cell CLL/lymphoma 11B using the numbering given in Swiss-Prot entry Q9C0K0 (GeneID 64919).
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCL11B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:0000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BCL11B Antibody - BSA Free

• B-cell CLL/lymphoma 11B (zinc finger protein)
• B-cell CLL/lymphoma 11B
• B-cell CLL/lymphoma 11B/T-cell receptor delta constant region fusion protein
• B-cell lymphoma/leukaemia 11B
• B-cell lymphoma/leukemia 11B
• BCL-11B
• BCL11B/TRDC fusion
• COUP-TF-interacting protein 2
• CTIP-2
• CTIP2ZNF856B
• hRit1
• hRIT1-alpha
• Radiation-induced tumor suppressor gene 1 protein
• RIT1
• zinc finger protein hRit1 alpha

Background

This gene encodes a C2H2-type zinc finger protein and is closely related to BCL11A, a gene whose translocation may be associated with B-cell malignancies. The specific function of this gene has not yet been determined. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BCL11B Antibody (NB600-264)(1)

Publication using NB600-264

• Matsumoto Yosuke, Nagoshi Hisao, Yoshida Mihoko et al. Expression of Master Regulators of T-cell, Helper T-cell and Follicular Helper T-cell Differentiation in Angioimmunoblastic T-cell Lymphoma. Internal Medicine (Tokyo, Japan) 2017-01-01 [PMID: 28943543] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: BCL11B
• Entrez:
  • 64919(Human)
• Uniprot:
  • Q9C0K0(Human)