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BCL11B Antibody

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Western Blot: BCL11B Antibody [NB600-263] - Detection of Human Bcl11b by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Bcl11b antibody NB600-263 used for WB at 0.066 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Biological Strategies: Western Blot: BCL11B Antibody [NB600-263] - Detection of Human Bcl11b by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. ...read more
Immunohistochemistry: BCL11B Antibody [NB600-263] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-Bcl11b used at a dilution of 1:1,000 (1ug/ml). Detection: DAB staining using ...read more
Immunoprecipitation: BCL11B Antibody [NB600-263] - Detection of Human Bcl11b on HeLa whole cell lysate using NB600-263. Bcl11b was also immunoprecipitated using affinity purified rabbit anti-Bcl11b antibodies NB600-262 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
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Biological Strategies

      

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BCL11B Antibody Summary

Immunogen
A synthetic peptide which maps to a region between residues 500 and 550 of human B-cell CLL/lymphoma 11B using the numbering given in Swiss-Prot entry Q9C0K0 (GeneID 64919).
Specificity
NB600-263 is specific for human Bcl-11b protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BCL11B
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:15000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1-5 ug/mg lysate
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:5,000 - 1:15,000 Immunoprecipitation: 1 to 5 ug/mg lysate Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BCL11B Antibody

  • B-cell CLL/lymphoma 11B (zinc finger protein)
  • B-cell CLL/lymphoma 11B
  • B-cell CLL/lymphoma 11B/T-cell receptor delta constant region fusion protein
  • B-cell lymphoma/leukaemia 11B
  • B-cell lymphoma/leukemia 11B
  • BCL-11B
  • BCL11B/TRDC fusion
  • COUP-TF-interacting protein 2
  • CTIP-2
  • CTIP2ZNF856B
  • hRit1
  • hRIT1-alpha
  • Radiation-induced tumor suppressor gene 1 protein
  • RIT1
  • zinc finger protein hRit1 alpha

Background

This gene encodes a C2H2-type zinc finger protein and is closely related to BCL11A, a gene whose translocation may be associated with B-cell malignancies. The specific function of this gene has not yet been determined. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunoprecipitation: Ctip1 Antibody [NB600-261] - Detection of Human Bcl11a on HeLa whole cell lysate using NB600-261. Bcl11a was also immunoprecipitated using affinity purified rabbit anti-Bcl11a antibodies NB600-258 and NB600-259.
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Bioinformatics

Gene Symbol BCL11B
Entrez
Uniprot