BCL11B Antibody - BSA Free

Western Blot: BCL11B Antibody [NB600-262] - Samples: Nuclear extract (5, 15 and 50 ug for WB, 1 mg for IP) from Jurkat cells. Antibodies: Affinity purified rabbit anti-Bcl11b antibody .

Immunoprecipitation: BCL11B Antibody [NB600-262] - Detection of Human Bcl11b on HeLa whole cell lysate using NB600-262. Bcl11b was also immunoprecipitated using affinity purified rabbit anti-Bcl11b antibodies NB600-263 and NB600-264.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

BCL11B Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit BCL11B Antibody - BSA Free (NB600-262) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-BCL11B Antibody: Cited in 7 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide which maps to a region between residues 1 and 50 of human B-cell CLL/lymphoma 11B using the numbering given in Swiss-Prot entry Q9C0K0 (GeneID 64919).
• Specificity: NB600-262 is specific for human Bcl-11b protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCL11B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. IHC reactivity reported in (PMID: 22028030).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22028030).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BCL11B Antibody - BSA Free

• B-cell CLL/lymphoma 11B (zinc finger protein)
• B-cell CLL/lymphoma 11B
• B-cell CLL/lymphoma 11B/T-cell receptor delta constant region fusion protein
• B-cell lymphoma/leukaemia 11B
• B-cell lymphoma/leukemia 11B
• BCL-11B
• BCL11B/TRDC fusion
• COUP-TF-interacting protein 2
• CTIP-2
• CTIP2ZNF856B
• hRit1
• hRIT1-alpha
• Radiation-induced tumor suppressor gene 1 protein
• RIT1
• zinc finger protein hRit1 alpha

Background

The bcl-11b gene encodes a C2H2-type zinc finger protein and is closely related to BCL11A, a gene whose translocation may be associated with B-cell malignancies. The specific function of this gene has not yet been determined. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BCL11B Antibody (NB600-262)(7)

Publications using NB600-262

• Inoue J, Ihara Y, Tsukamoto D et al. Identification of BCL11B as a regulator of adipogenesis Sci Rep 2016-09-02 [PMID: 27586877] (IP, Human)
• Fujimoto R, Ozawa T, Itoyama T et al. HELIOS-BCL11B fusion gene involvement in a t(2;14)(q34;q32) in an adult T-cell leukemia patient. Cancer Genet 2012-07-01 [PMID: 22867996]
• Le Douce V, Colin L, Redel L et al. LSD1 cooperates with CTIP2 to promote HIV-1 transcriptional silencing. Nucleic Acids Res 2012-03-01 [PMID: 22067449]
• Watanabe K, Takebayashi H, Bepari AK et al. Dpy19l1, a multi-transmembrane protein, regulates the radial migration of glutamatergic neurons in the developing cerebral cortex. Development 2011-11-01 [PMID: 22028030] (IF/IHC, Mouse)
• Tang B, Di Lena P, Schaffer L et al. Genome-wide identification of Bcl11b gene targets reveals role in brain-derived neurotrophic factor signaling. PLoS One 2011-01-01 [PMID: 21912641]
• Zhang S, Rozell M, Verma RK et al. Antigen-specific clonal expansion and cytolytic effector function of CD8+ T lymphocytes depend on the transcription factor Bcl11b. J Exp Med 2010-08-01 [PMID: 20660613]
• Cismasiu VB, Ghanta S, Duque J et al. BCL11B participates in the activation of IL2 gene expression in CD4+ T lymphocytes. Blood 2006-10-01 [PMID: 16809611]

Bioinformatics

• Gene Symbol: BCL11B
• Entrez:
  • 64919(Human)
• Uniprot:
  • Q9C0K0(Human)