BCL11B Antibody (1F8G8) - BSA Free

Western Blot: BCL11B Antibody (1F8G8) [NBP2-61702] - Analysis using BCL11B mAb against human BCL11B (AA: 1-150) recombinant protein. (Expected MW is 42.3 kDa)

Immunocytochemistry/Immunofluorescence: BCL11B Antibody (1F8G8) [NBP2-61702] - Analysis of Hela cells using BCL11B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry-Paraffin: BCL11B Antibody (1F8G8) [NBP2-61702] - Analysis of stomach cancer tissues using BCL11B mouse mAb with DAB staining.

Flow Cytometry: BCL11B Antibody (1F8G8) [NBP2-61702] - Analysis of Jurkat cells using BCL11B mouse mAb (green) and negative control (red).

Western Blot: BCL11B Antibody (1F8G8) [NBP2-61702] - Analysis using BCL11B mAb against HEK293 (1) and BCL11B (AA: 1-150)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry-Paraffin: BCL11B Antibody (1F8G8) [NBP2-61702] - Analysis of colon cancer tissues using BCL11B mouse mAb with DAB staining.

Flow Cytometry: BCL11B Antibody (1F8G8) [NBP2-61702] - Analysis of Hela cells using BCL11B mouse mAb (green) and negative control (red).

ELISA: BCL11B Antibody (1F8G8) [NBP2-61702] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 1F8G8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

BCL11B Antibody (1F8G8) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human BCL11B (AA: 1-150) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BCL11B
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:50-1:250
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 95.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for BCL11B Antibody (1F8G8) - BSA Free

• B-cell CLL/lymphoma 11B (zinc finger protein)
• B-cell CLL/lymphoma 11B
• B-cell CLL/lymphoma 11B/T-cell receptor delta constant region fusion protein
• B-cell lymphoma/leukaemia 11B
• B-cell lymphoma/leukemia 11B
• BCL-11B
• BCL11B/TRDC fusion
• COUP-TF-interacting protein 2
• CTIP-2
• CTIP2ZNF856B
• hRit1
• hRIT1-alpha
• Radiation-induced tumor suppressor gene 1 protein
• RIT1
• zinc finger protein hRit1 alpha

Background

This gene encodes a C2H2-type zinc finger protein and is closely related to BCL11A, a gene whose translocation may be associated with B-cell malignancies. Although the specific function of this gene has not been determined, the encoded protein is known to be a transcriptional repressor, and is regulated by the NURD nucleosome remodeling and histone deacetylase complex. Four alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BCL11B Antibody (NBP2-61702)(2)

Publications using NBP2-61702

• Tristan C, Ormanoglu P, Slamecka J et al. Robotic High-Throughput Biomanufacturing and Functional Differentiation of Human Pluripotent Stem Cells Stem Cell Reports 2021-12-03 [PMID: 34861164]
• Tristan CA, Ormanoglu P, Slamecka J et al. Robotic High-Throughput Biomanufacturing and Functional Differentiation of Human Pluripotent Stem Cells bioRxiv 2020-08-03 [PMID: 32793899] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: BCL11B