Bcl-xL Antibody (3E2-2A5)

Images

 
Western Blot: Bcl-xL Antibody (3E2-2A5) [H00000598-M01] - BCL2L1 monoclonal antibody (M01), clone 3E2-2A5 Analysis of BCL2L1 expression in A-431.
Immunocytochemistry/ Immunofluorescence: Bcl-xL Antibody (3E2-2A5) [H00000598-M01] - Analysis of monoclonal antibody to BCL2L1 on A-431 cell. Antibody concentration 10 ug/ml.
Western Blot: Bcl-xL Antibody (3E2-2A5) [H00000598-M01] - Analysis of BCL2L1 expression in transfected 293T cell line by BCL2L1 monoclonal antibody (M01), clone 3E2-2A5.Lane 1: BCL2L1 transfected lysate (Predicted MW: ...read more
Proximity Ligation Assay: Bcl-xL Antibody (3E2-2A5) [H00000598-M01] - Analysis of protein-protein interactions between BID and BCL2L1. HeLa cells were stained with anti-BID rabbit purified polyclonal 1:1200 and ...read more
Sandwich ELISA: Bcl-xL Antibody (3E2-2A5) [H00000598-M01] - Detection limit for recombinant GST tagged BCL2L1 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, PLA
Clone
3E2-2A5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Bcl-xL Antibody (3E2-2A5) Summary

Immunogen
BCL2L1 (AAH19307, 1 a.a. - 233 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIAAWMATYLNDHLEPWIQENGGWDTFVELYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK
Specificity
BCL2L1 - BCL2-like 1
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BCL2L1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Proximity Ligation Assay
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Publications
Read Publication using H00000598-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Bcl-xL Antibody (3E2-2A5)

  • Apoptosis regulator Bcl-X
  • bcl2-L-1
  • BCL2-like 1
  • bcl-2-like protein 1
  • BCLXBCL2LBcl-X
  • BclxL
  • Bcl-xL
  • BCL-XL/S
  • BCLXS
  • bcl-xS
  • DKFZp781P2092

Background

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Bcl-xL Antibody (H00000598-M01)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Blogs on Bcl-xL.

BAG3 - Hsp70 is my friend!
The BAG proteins are a large family of chaperone regulators governing a wide range of cell processes such as proliferation, survival, stress response, tumorigenesis, neuronal differentiation, growth arrest and apoptosis as reviewed in Takayama et al1....  Read full blog post.

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Bioinformatics

Gene Symbol BCL2L1
Entrez
OMIM
Uniprot