Bcl-9 Antibody [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 405

Bcl-9 Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human Bcl-9
  Met1009-Gly1328
  Accession # O00512
• Specificity: Detects endogenous human Bcl-9 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Bcl-9 Antibody [Alexa Fluor® 405]

• B-cell CLL/lymphoma 9 protein
• B-cell CLL/lymphoma 9
• B-cell lymphoma 9 protein
• Bcl9
• Bcl-9
• BCL9L
• LGS
• MGC131591
• Protein legless homolog

Background

Bcl-9 (B-cell lymphoma 9; also Protein legless homolog) is a transcriptional regulator that belongs to the Bcl-9 family of proteins. It is expressed in multiple tissues and serves to recruit Pygopus to the Wnt-pathway beta -catenin-TCF complex in the nucleus. Bcl-9 and Bcl-9-2 are considered evolutionary duplicates of Legless that perform the same task with different regulation. Human Bcl-9 is 1426 amino acids (aa) in length. It contains one phosphothreonine and three phosphoserine sites that have been identified so far, two poly-Pro regions (aa 514‑517 and 970‑973), and one poly-Ala segment (aa 900‑903). There is one potential alternate start site at Met27, and a variant isoform exists that shows a four aa substitution for aa 1391‑1426. Over aa 1009‑1328, human Bcl-9 is 96% aa identical to mouse Bcl-9.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.